目的：观察中药驻景方对氪激光诱导的病理性近视脉络膜新生血管VEGF表达的影响，探讨中药驻景方对病理性近视脉络膜新生血管的干预作用。

方法：将3周龄雌性三色豚鼠45只随机选出15只为空白对照组，剩余豚鼠均带头套右眼形觉剥夺诱导病理性近视，A超检影(诱导失败者剔除出组)并随机分为模型组、中药组，双眼行氪激光光凝。中药组于光凝后第2d开始连续灌胃21d，3.285g/(kg·d)，每次1.5mL，1次/d。21d后行脉络膜铺片、HE染色、免疫荧光、免疫组织化学观察。

结果：形觉剥夺4wk后右眼均诱导出高度近视，且眼轴较左眼增长； 脉络膜铺片及免疫荧光示模型组右眼CNV面积及视网膜VEGF表达均明显高于左眼(P<0.01)，中药组右眼CNV的面积及VEGF的表达较模型组右眼减少(P<0.01)； 免疫组织化学结果显示，中药组右眼VEGF的平均光密度值(0.0589±0.0146)较模型组右眼(0.0972±0.0507)减少(P<0.01)。

结论：中药驻景方可抑制氪激光诱导的病理性近视脉络膜新生血管的生长及VEGF的表达。

AIM: To observe the effect of traditional medicine to the expression of vascular endothelium growth factor(VEGF), and explore the intervention effect to the pathological myopia with choroidal neovascularization(CNV)through experimental model of lasers -induced CNV in guinea pigs with pathological myopia induced by form deprivation.

METHODS: Fifteen of the forty-five 3-week old female guinea pigs were randomly selected as the blank control group, the rest 30 guinea pigs were using facemask to induce high myopia in 4 weeks, then randomly assigned to model group and treatment group, then induced CNV by different wavelength laser. The treatment group gavaged with modified Zhu Jing prescriptions while the control group was gavaged with distill water. 21 days after treatment, histopathologic choroidal flat mounts, HE stain,immunofluorescence stain and immuno- histochemistry for VEGF in the experimental eyes and control eyes were performed.

RESULTS:All the 30 guinea pigs were successfully induced high myopia and the axial length of the right eyes were significantly greater than the non-facemask eyes and the eyes of control group. Immunofluorescence stain and immunohistochemistry for VEGF showed that the expression of VEGF of the treatment group was less than the model group. The average optical density(AOD)of the form deprivation eye in the treatment group(0.0589±0.0146)was higher than that in the model group(0.0972±0.0507)(P<0.01).

CONCLUSION: Modified Zhu Jing prescriptions has a positive effect to the reduction of the VEGF expression in the CNV with pathological myopia and thus it can inhibit the growth of CNV.

国家自然科学基金项目(No.81072843)