方法：制备细胞8-DC，GFP-DC及IL-10-GFP-DC。55只SD受体大鼠，随机抽取6只6眼，作为阴性对照组，其余随机分成4组：阳性对照组(术前3d受体大鼠尾静脉注射1mL PBS)、8-DC组、GFP-DC组、IL-10-GFP-DC组，分别于术前3d受体大鼠尾静脉注射1mL已制备的细胞悬液(8-DC，GFP-DC，IL-10-GFP-DC)。以Wistar大鼠为供体建立角膜移植实验模型。术后观察各组大鼠的角膜植片状态，并于术后第14d采用ELISA方法检测大鼠房水中IL-4和IFN-γ因子的表达。

结果：IL-10-GFP-DC组角膜植片的存活时间显著延长。IL-10-GFP-DC组细胞因子IL-4表达较其他各组高，而IFN-γ的表达较其他各组低，差异具有统计学意义。

结论：外源性IL-10基因转染的未成熟树突状细胞能抑制角膜移植排斥反应，诱导免疫耐受的形成。其中干扰大鼠房水中细胞因子IL-4和IFN-γ的表达是诱导角膜移植免疫耐受的机制之一。

METHODS: Cells of 8-DC, GFP-DC and IL-10-GFP-DC were prepared. Totally 55 SD rats were randomly selected 6(6 eyes), as a negative control group, the rest were randomly divided into 4 groups: positive control group, injected of 1mL PBS by receptor tail vein 3 days before surgery, and 8-DC group, GFP-DC group, IL-10-GFP-DC group, respectively, injected of 1mL cell suspension(8-DC, GFP-DC and IL-10-GFP-DC)have been prepared by receptor tail vein 3 days before surgery. Wistar rats as donor, keratoplasty was performed. The rats corneal graft status was examined after operation, and ELISA method was used to detect the expression of IL-4, IFN- γ in aqueous humor at the fourteenth day after operation.

RESULTS: The survival time of corneal graft in group IL-10-GFP-DC was significantly prolonged. Expression of IL-4 in group IL-10-GFP-DC cytokines was higher than that in other groups, while the expression of IFN- γ was lower than other groups, the difference was statistically significant.

CONCLUSION: Exogenous IL-10 gene transfection to immature dendritic cells could inhibit corneal allograft rejection, and induce the formation of immune tolerance. The disturbance over the expression of cytokines IL-4, IFN- γ in aqueous humor is one of the mechanisms for the induction of immune tolerance in corneal transplantation.