[关键词]
[摘要]
目的：通过观察大鼠角膜损伤愈合过程中骨形成蛋白-7(bone morphogenetic protein 7，BMP-7)的表达规律来探讨其在大鼠角膜损伤愈合过程中可能发挥的作用。
方法：雄性Wistar大鼠42只，随机分为7组，每组6只，其中一组为正常对照组，动物模型组均取右眼制造角膜针刺损伤模型。于损伤后各时间点(6h； 1，3，5，7，14d)取角膜标本，HE染色行组织病理学检查，免疫组织化学染色观察BMP-7的表达与分布，计算机图像分析系统对结果进行分析。
结果：鼠角膜上皮BMP-7的表达于损伤后1，3d逐渐升高(P<0.05)； 损伤后5d时表达逐渐降低，至损伤后14d时表达恢复正常。角膜基质层及角膜内皮层中BMP-7的表达未见明显变化。
结论：BMP-7在大鼠角膜上皮层均有表达，可能作为负性的生长因子参与角膜损伤愈合过程。
[Key word]
[Abstract]
AIM: To observe the expression of bone morphogenetic protein 7(BMP-7)during corneal wound healing in rats, and to discuss the effects of BMP-7 on corneal wound healing.
METHODS: Totally 42 male Wistar rats were divided into seven groups, six rats in each group and one of the groups as control group. The experimental groups were made into cornea model by needle puncture at their corneas of right eyes. The corneas were surgically removed at six different time points(6h, 1d, 3d, 5d, 7d, 14d)after operation. The localization and expression of BMP-7 was determined by immunohistochemical methods and computer image analysis.
RESULTS: The expression of BMP-7 of experimental groups gradually rose on the 1st day and 3rd day in the injured corneal epithelium after operation and significant difference(P< 0.05). Then it decreased gradually on the 5th day. And it reached the normal level on the 14th day.
CONCLUSION: BMP-7 is expressed in cornea of rats and may play a negative role on corneal wound healing after injury.
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