方法：回顾性病例系列研究。将150例300眼近视眼按预设角膜瓣厚度分为100μm组(204眼)和110μm组(96眼)，角膜瓣直径7.9和8.3mm。将术前设定的角膜瓣直径和厚度与术中、术后测量的实际结果进行差异性分析，对相关影响因素进行多元逐步回归分析。

结果：100μm组和110μm组的角膜瓣厚度测量值分别为103.11±4.07，113.35±5.71μm，与目标值比较，差异无统计学意义(t=0.396，1.284，P>0.05)。左右眼差异无统计学意义(t_100μm =-0.901，t_110μm =-0.490； P>0.05)。100μm组和110μm组患者的角膜厚度测量值分别为533.52±20.89，530.24±25.43μm，年龄分别为23.80±6.71，21.56± 5.08岁，角膜中央最大K值分别为43.46±1.39，43.44±1.38D，角膜瓣直径分别为8.46±0.47，8.72±0.43mm，眼压分别为16.02±2.51，16.66±2.21mmHg(1mmHg=0.133kPa)。两组角膜瓣厚度与角膜瓣直径(r=0.003，0.018)、术前年龄(r=0.022，0.050)、角膜厚度(r=0.051，0.101)、角膜中央最大K值(r=-0.048，-0.136)及眼压(r=-0.113，0.047)均无相关性(P>0.05)。预设角膜瓣直径为7.9，8.3mm的角膜厚度分别为536.88±27.99，529.83±33.52μm，角膜中央最大K值分别为43.48±1.19，41.88±1.25D。相关性分析显示：术前角膜中央最大K值与角膜瓣直径呈正相关(r=0.359，0.532； P=0.01，0.007 <0.05)。

结论：VisuMax飞秒激光仪制作角膜瓣厚度预测性好，不受角膜瓣直径、角膜厚度、角膜中央最大K值、年龄、眼压等因素的影响。

METHODS: This retrospective case series study included 300 eyes of 150 consecutive patients. The eyes were assigned to two groups according to intended flap thickness, 100μm(204 eyes)and 110μm(96 eyes), which created with the VisuMax femtosecond laser. Intended flap diameters were 7.9mm and 8.3mm. Difference analysis of flap diameter and intended diameter as well as flap thickness and intended thickness were made. The data were analyzed with SPSS to sum up a multiple stepwise regression formula that could express their quantitative relationship.

RESULTS: The 100μm flap group had an average flap thickness of 103.11±4.07μm, while for the 110μm group the average flap thickness was 113.35±5.71μm. The difference between right and left eyes was not statistically significant(t_100μm =-0.901, t _110μm=-0.490; P>0.05). Corneal flap thickness was not related to flap diameter(r=0.003, 0.018; P>0.05), preoperative patient age(r=0.022, 0.050; P>0.05), corneal thickness(r=0.051, 0.101; P>0.05), keratometric value K(r=-0.048, -0.136; P>0.05)or intraocular pressure(r=-0.113, 0.047; P>0.05). Preoperative corneal keratometric value K was positively correlated with corneal flap diameter(r=0.359, 0.532; P=0.01, 0.007<0.05).

CONCLUSION:The LASIK flap creation with the VisuMax femtosecond laser has relatively good predictability. There is no influencing factor for flap thickness.