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[摘要]
目的：观察抗血小板源性生长因子受体ɑ(PDGFR-ɑ)抗体对铁锈诱导的人视网膜色素上皮细胞(hRPE)增殖活性的影响。
方法：采用细胞体外培养技术，用铁锈建立hRPE细胞增殖分化模型。设置空白组、铁锈组、抗PDGFR-ɑ抗体(1，10，50，100μg/mL)处理组，分别作用0，12，24，48h后采用MTT比色法测定hRPE细胞的抑制率。
结果：加入抗PDGFR-ɑ抗体后hRPE细胞增殖活性在一定浓度范围内随着抗体浓度增加而下降，而50μg/mL抗PDGFR-ɑ抗体为抑制hRPE细胞增殖的最佳浓度，其抑制率为42.44%。
结论：抗PDGFR-ɑ抗体可以抑制hRPE细胞的增殖活性。
[Key word]
[Abstract]
AIM: To study the effect of platelet derived growth factor ɑ receptor(PDGFR-ɑ)antibody on the rust-induced human retinal pigment epithelial cell(hRPE)proliferation.
METHODS: In vitro rust hRPE cells proliferation differentiation model was established. Blank control group, rust group, PDGFR-ɑ antibody(1, 10, 50, and 100μg/mL)treatment group were established. The effect of anti-PDGFR-ɑ antibody on the growth of RPE was determined by MTT colorimetric assay after 0, 12, 24, 48h,and the inhibition rates were calculated accordingly.
RESULTS: After adding the anti-PDGFR-ɑ antibody, hRPE cells proliferation activity was decreased at certain concentration as the antibody concentration increased, and 50μg/mL anti-PDGFR-ɑ antibody was the optimum concentration to inhibit hRPE cells proliferation, the inhibitory rate of hRPE was 42.44%.
CONCLUSION: The anti-PDGFR-ɑ antibody shows strong inhibitory effect on the growth of cultured hRPE.
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