Adipose-derived stem cells(ADSCs)are multipotent population of cells with multipotential differentiation capability, which can differentiate into mesoderm including adipocyte osteoblasts and chondroblasts in specific conditions, even the endoderm cells like hepatocyte and and ectoderm cells like neurocyte. Besides, ADSC has many advantages like easy access and light damage to selected area, which enables it to become a hotspot in tissue repair area. This paper made a classified summary on the biological characteristics of corneal epithelium and its application in research to offer beneficial hints to the research of ADSC application in ophthalmology.