方法：患者40眼行FS200飞秒激光制瓣LASIK手术，40眼行VisuMax飞秒激光制瓣LASIK手术。观察术前及术后1wk； 1，3mo的泪膜破裂时间(tear film break-up time ，TBUT)、基础泪液分泌试验(SchirmerⅠtest，SⅠt)以及角膜荧光素染色(fluorescent，FL)检查。多个时间点的比较采用重复测量设计的方差分析或Kruskal—Wallis秩和检验，两组间的比较采用两独立样本t检验或Wilcoxon符号秩和检验。

结果：FS200组术后的TBUT有较VisuMax组更少的趋势，但两组间的各项观察指标均没有统计学意义的差别。术后各时间点两组的TBUT值均低于术前(F=100.37，P<0.01； F=112.25，P<0.01)。两组在术后各时间点SⅠt明显低于术前(F=42.15，P<0.01； F=37.27，P<0.01)。术后1wk时FS200的FL(Z=4.28，P<0.01)和VisuMax组(Z=4.83，P<0.01)均较术前增多，但在1mo时恢复到术前水平。

结论：研究表明两种飞秒激光制瓣ASIK术后的TBUT、SⅠt以及FL的变化一致，但是VisuMax飞秒制瓣LASIK术后的TBUT有比FS200飞秒制瓣LASIK手术更快恢复的趋势。

METHODS:Forty eyes underwent FS200 and 40 eyes underwent VisuMax. The tear film break-up time(TBUT), Schirmer I test(SⅠt)and fluorescein staining were assessed preoperatively at 1wk; 1 and 3mo postoperatively. The analysis of variance(ANOVA)for repeat measurements or Kruskal—Wallis ranks test were used. For the differences between FS200 and VisuMax-operated eyes, a independent-samples t test or Wilcoxon matched-samples rank sum test were used.

RESULTS: There were no statistical differences in any clinical outcome measure between the two kinds of lasers(P<0.05), although there was a trend towards slightly reductions for TBUT in FS200-operated eyes. TBUT of the two groups eyes showed the same reductions(F=100.37, P<0.01; F=112.25, P<0.01)when compared preoperative levels to 1wk; 1 and 3mo postoperatively levels. Moreover, there were obvious decreases of the Schirmer Ⅰ test were observed on any visit after the operation for both groups(F=42.15, P<0.01; F=37.27, P<0.01). Furthermore, corneal staining was significantly increased at 1wk postoperatively(FS200 Z=4.88, P<0.01; VisuMax Z=4.28, P<0.01)after LASIK compared to preoperative conditions, and recovered to the level of preoperative at 1mo postoperatively.

CONCLUSION: This study shows that changes in TBUT,Schirmer I test and corneal staining are statistically similar in LASIK using FS200 and VisuMax femtosecond lasers for flap creation. However, the VisuMax-operated eyes of TBUT after LASIK have faster recovery than in FS200 eyes.