方法：免疫组织化学方法(PV)检测翼状胬肉(72眼)及胬肉旁结膜组织(30眼)中TNF-α及其受体P55的表达，分析与翼状胬肉临床病理特征的关系。

结果：TNF-α在翼状胬肉和胬肉旁结膜组织中的阳性表达率分别为65.3%(47/72)，26.7%(8/30)，差异有显著统计学意义(χ²=12.706，P<0.01)。P55在翼状胬肉和胬肉旁结膜组织中的阳性表达率分别为56.9%(41/72)及16.7%(5/30)，差异有显著意义(χ²=13.875，P<0.01)。TNF-α在复发性胬肉的阳性表达率高于初发性胬肉(χ²=6.547，P=0.011)，表达强度差异比较无统计学意义(F=1.288，P=0.393)； 进展期胬肉阳性表达率高于静止期胬肉(χ²=4.082，P=0.043)，表达强度比较无统计学意义(F=0.489，P=0.708)。P55在复发性胬肉的阳性表达率高于初发性胬肉(χ²=9.907，P=0.002)，表达强度比较无统计学意义(F=1.175，P=0.424)； 进展期胬肉的阳性表达率明显高于静止期胬肉(χ²=11.140，P=0.001)，表达强度比较无统计学意义(F=0.665，P=0.621)。

结论：TNF-α及P55在翼状胬肉中的阳性表达率随着发病状态和临床分期的进展而变化。TNF-α及P55表达与临床分类、临床分期、患者工作生存状况相关。TNF-α及P55在翼状胬肉不同发病状态及临床分期的表达强度无明显差异。

METHODS: Immunohistochemistical staining method(PV)was adopted to detect the expression of TNF-α and receptor I in pterygium(72 eyes)and para-pterygium conjunctival tissue(30 eyes). The relationship between the expression and clinical-pathological parameters was also analyzed.

RESULTS: The positive rates of TNF-α were 65.3%(47/72), 26.7%(8/30)in pterygium and para-pterygium conjunctival tissue. The positive expression of TNF-α had statistic difference between the two groups(χ²=12.706, P<0.01). The positive rates of TNF-α receptor I were 56.9%(41/72), 16.7%(5/30)in pterygium and para-pterygium conjunctival tissue. The positive expression of P55 had statistic difference between the two groups(χ²=13.875, P<0.01). The positive rate of TNF-α in recurrent pterygium group was higher than primary pterygium group(χ²=6.547, P=0.011). There had no statistically significance of the expression intensity between the two groups(F=1.288, P=0.393); the positive rate in advanced pterygium group was higher than quiescent pterygium group(χ²=4.082, P=0.043). The expression intensity had no statistically significance between the two groups(F=0.489, P=0.708). The positive rate of P55 in recurrent pterygium group was higher than primary pterygium group(χ²=9.907, P=0.002). There had no statistically significance of the two group's expression intensity(F=1.175, P=0.424); the positive rate in advanced pterygium group was higher than in quiescent pterygium group(χ²=11.140, P=0.001). The expression intensity had no statistically significance between the two groups(F=0.665, P=0.621).

CONCLUSION:The expression of TNF-α and P55 are changing according to the development of clinical staging and onset. The expression of TNF-α and P55 may be related to clinical classification, staging and patient's working conditions of pterygium. There has no significant difference expression intensity of TNF-α and P55 in clinical staging and onset of pterygium.