方法：将150只雄性日本大耳白兔随机分为正常组(A组)、假手术组(B组)、模型组(C组)、雄激素对照治疗组(D组)和菊花总黄酮治疗组(E组)。C组、D组、E组行双侧去势术建立白兔干眼症模型。E组白兔用菊花总黄酮灌胃治疗，D组用雄激素肌肉注射，A组、B组、C组用生理盐水灌胃。分别于治疗后1，3，5mo每组处死10只，取材用与相关指标检测。全部白兔行SchirmerⅠ试验，并检测泪膜破裂时间，处死后取泪腺组织，采用免疫组织化学法检测泪腺组织中凋亡相关基因蛋白Fas、FasL的表达，并计数凋亡细胞数量。

结果：E组SchirmerⅠ试验测量所得滤纸湿长明显高于C组(P<0.01)，泪膜破裂时间明显长于C组(P<0.01)。E组治疗1，3，5mo后，泪腺导管及腺泡上皮细胞中Fas阳性表达的细胞数均明显低于C组，FasL阳性表达的细胞数均明显高于C组，细胞凋亡数量均明显低于C组(P<0.01)。

结论：菊花总黄酮中主要成分为黄酮类物质，可显著抑制雄激素水平降低后白兔干眼症的发生，抑制泪腺细胞凋亡，维持泪腺基础分泌量和泪膜的稳定性。

METHODS: Totally 150 male Japanses white rabbits were divided into blank group(group A), sham-operated group(group B), model group(group C), androgen control treatment group(group D), and total flavonoid of chrysanthemum treatment group(group E). The dry eye model was established with orchiectomy on group C, D and E. Rabbits in group E were treated with total flavonoid of chrysanthemum. Rabbits in group D were treated with androgen intramuscular injection. Rabbits in the group A, group B, group C was treated with normal saline. All rabbits were detected with Schirmer's Ⅰ test and tear break-up time(BUT). Fas, FasL were checked on immunohistochemistry.

RESULTS: The Schirmer's I test values of group E was significantly higher than that of group C(P<0.01)and the BUT value of group E was significantly longer than that of group C(P<0.01). The quantity of positive expression of Fas in glandular tube cell and acinar epithelial celland apoptosis cells of group E after treatment at 1, 3, 5mo were significantly lower than that of group C, cell population of the positive expression of FasL was obviously higher than that of group C(P<0.01).

CONCLUSION:The main component of chrysanthemum is flavonoid, which could significantly inhibit happening of dry eye in rabbit after androgen level lowered and lacrimal gland apoptosis and keep basic tears secretory volume and tear film stability.