方法：对24例24眼角膜病变患者实施飞秒激光穿透性角膜移植手术，对术前术后角膜内皮细胞密度、视觉质量进行分析比较。

结果：术后1wk，角膜移植片透明者21眼(87.5%)，移植片轻度混浊者3眼(12.5%)。视力≥0.5者18眼(75.0%)，0.2～0.4者6眼(25.0%)。术后3mo，角膜散光2.25～3.09(平均2.16±0.21)D，较常规角膜移术后散光产生(平均3.67±0.38D)有降低，有显著性差异(P<0.05)； 比较患者术前和术后的视力及散光均有差异(P均<0.05)。

结论：飞秒激光辅助穿透性角膜移植手术根据不同角膜内皮细胞计数设计不同角膜瓣形状，术后可以显著提高患者的视觉质量，比传统穿透性角膜移植散光明显减少，切口精确整齐且可以选择个体化形状，具有更多的手术优点。

METHODS: Twenty-four cases(24 eyes)with corneal lesions were performed with femtosecond laser assisted penetrating keratoplasty. Preoperative and postoperative endothelial cell density and visual quality were compared.

RESULTS: One week after operation, corneal grafts were clear in 21 eyes(87.5%), mild cloudy in 3 eyes(12.5%); visual acuity ≥0.5 in 18 eyes(75.0%), 0.2～0.4 in 6 eyes(25.0%). After 3mo the mean corneal astigmatism was 2.16±0.21D(range 2.25～3.09D). Compared to conventional penetrating keratoplasty which mean corneal astigmatism was average 3.67±0.38D after operation, there was significant difference between two groups(P<0.05). There were significant differences between preoperative and postoperative visual acuity and astigmatism(both P<0.05).

CONCLUSION: Femtosecond laser assisted penetrating corneal transplantation operation can improve patient's visual quality. And compared to traditional penetrating keratoplasty astigmatism decreased significantly, incision can be made in individual shape more precisely and neatly.