方法：用头套形觉剥夺右眼的方法诱导近视动物模型。实验前后检测豚鼠双眼屈光状态及眼轴长度。HE染色后观察后极部视网膜形态学改变，通过免疫组化对TGF-β₂在视网膜内表达进行定性及半定量测定。

结果：实验组视网膜变薄，且内核层的胞核层数较对照眼少，视网膜外核层细胞核变小、变圆，排列不均，视细胞层外节内节排列紊乱。免疫组化检查示实验组视网膜TGF-β₂表达明显下调。

结论：头套遮盖效果明显，是一种诱导形觉剥夺性近视简便、稳定的方法。形觉剥夺性近视眼的视网膜产生了一系列的退行性改变。TGF-β₂可能是最终导致形觉剥夺性近视形成的重要因素之一。

METHODS: A facemask was worn on the right eyes of guinea pigs to develop form deprivation myopia. Before and after experiment, refraction was measured using retinoscope, and ocular axial was determined by A-scan ultrasonography. Morphologic alternations of the retina were observed by light microsope. The expression of TGF-β₂ in the retina was determined by immunohistochemistry.

RESULTS:Retina of myopic eyes became thinner, and inner nuclear layer of retina had few layers, nuclear of outer nuclear layer became smaller and circular, at the same time, they arranged in disorder. The immunohistochemisty results showed that expression of TGF-β₂ in model eyes was lower than that in control eyes(P<0.05).

CONCLUSION: Covering eyes with facemasks is effective, simple and convenient in making myopic model. The retina of the model eye appeares degeneration. TGF-β₂ plays an important role in the course of development in form deprivation myopia.