[关键词]
[摘要]
目的：研究曲古抑菌素A(trichostatin A，TSA)对体外培养的人Tenon囊成纤维细胞(human Tenon capsule fibroblast，HTF)增殖能力以及组蛋白去乙酰化酶1(histone deacetylase 1，HDAC1)和HDAC2蛋白表达的影响。
方法：取青光眼滤过术中Tenon囊组织进行HTF体外培养。选取第3～6代细胞进行实验。设置空白对照组及TSA组(600nmol/L TSA加入培养液中)，分别于培养后1，2，3d应用 MTT法检测细胞活力变化； 以600nmol/L TSA处理HTF 2d后，Western blot法检测细胞中HDAC1和HDAC2蛋白的表达。
结果：与对照组比较，TSA作用1d后HTF活力下降，呈时间依赖性，具有统计学差异(P<0.05)。TSA处理HTF后2d，Western blot法检测HDAC1和HDAC2蛋白表达受到明显抑制。
结论：TSA可以通过抑制HDAC1和HDAC2的表达量，抑制人Tenon囊成纤维细胞增殖，从而减少术后结膜瘢痕形成可能。
[Key word]
[Abstract]
AIM:To investigate the effect of trichostatin A(TSA)on cell proliferation and the expressions of histone deacetylase 1(HDAC1)and HDAC2 in cultured human Tenon capsule fibroblast(HTF).
METHODS: Human Tenon capsule fibroblasts were cultured in vitro after glaucoma filtration surgery. The third to sixth passage of cell were treated by 600nmol/L TSA or none. Cell viability measured by MTT assay after 1, 2 and 3d respectively. The expressions of HDAC1 and HDAC2 were analyzed by Western blot 2d after TSA treatment.
RESULTS: Compared to the control, cell viability decreased significantly after treatment with TSA at 1d(P<0.05), presented time-dependent manner. The expression of HDAC1 and HDAC2 significantly reduced in TSA-treated HTF compared with control cells at 2d after TSA treatment.
CONCLUSION: TSA inhibits the proliferation of Tenon capsule fibroblast by inhibiting the expression of HDAC1 and HDAC2, and reduces subconjunctival scar formation.
[中图分类号]
[基金项目]
陕西省教育厅专项科研计划项目(No.2013JK0785)
