[关键词]
[摘要]
目的：检测缺氧诱导因子(HIF-1α)和干细胞标志物(Pax6)在患者翼状胬肉组织中的表达，比较其与正常结膜组织中的变化，以探讨HIF-1α和Pax6的表达与翼状胬肉之间的关系。
方法：选取35例翼状胬肉患者切除的翼状胬肉组织为实验组，同时选取10例正常人结膜组织为对照组，使用常规免疫组织荧光染色法对HIF-1α和 Pax6的表达进行检测并与染色进行对照。
结果：实验组35例组织中HIF-1α的阳性表达率为66%(23/35)，正常对照组10例组织中HIF-1α的阳性表达率仅为10%(1/10)，HIF-1α表达在正常球结膜与翼状胬肉两组间的表达有统计学差异(P<0.05)。正常人球结膜Pax6在组织各层细胞核内表达，而Pax6在翼状胬肉组织中表达下降甚至未见表达，两组比较有统计学差异(P<0.05)。
结论：HIF-1α在翼状胬肉中高表达，提示它可能参与了翼状胬肉的病理过程； Pax6基因在翼状胬肉上皮细胞中表达下调，提示翼状胬肉上皮细胞病理过程属于鳞状上皮化生类型。
[Key word]
[Abstract]
AIM: To study the expression of hypoxia-inducible factor(HIF)and Pax6 in pterygium and investigate the relationship between expression of HIF and Pax6 in pterygium.
METHODS:Thirty-five patients with pterygium who underwent simple excision and 10 cases of normal conjunctival tissues were enrolled in this study. Immunofluorescence was performed in pterygia tissue and normal conjunctiva, detecting the expression of HIF-1α and Pax6.
RESULTS: The positive expression rate of HIF-1α was 66%(23/35)and 10%(1/10)in pterygia tissue and normal conjunctiva, respectively. The difference of expression between pterygia tissue and normal conjunctiva was statistically significant(P<0.05). Expression of Pax6 was full-thickness nuclear in normal conjunctiva; however, expression of Pax6 decreased and even was negative in pterygium. The difference of expression in two groups was statistically significant(P<0.05).
CONCLUSION: HIF-1α is highly expressed in pterygium, suggesting that it may be involved in the occurrence and development of pterygium. Pax6 gene downregulated in pterygium epithelial cells, suggesting that epithelial cells are squamous metaplasia.
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