方法：收集2010-01/2012-12河北邢台眼科医院诊治的急性细菌性结膜炎患者的结膜囊分泌物进行细菌学检查，并对致病菌种类及药敏试验结果进行分析。

结果：检标本186例中，培养阳性菌150例(80.6%)，其中革兰氏阳性菌127例(84.7%)，占前三位的分别是表皮葡萄球菌(28.0%)，人葡萄球菌(10.0%)，溶血葡萄球菌(4.0%)。革兰氏阴性菌23例(15.3%)，前四位的分别是：大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌、阴沟肠杆菌，均为2.0%。革兰氏阳性菌除对万古霉素敏感率为100%外，对其他抗菌药物均有不同程度的耐药性。革兰氏阴性菌中敏感率最高的是头孢哌酮(87.0%)。

结论：表皮葡萄球菌等条件致病菌已成为本地区急性细菌性结膜炎的主要致病菌。无论革兰阳性菌还是革兰阴性菌对临床常用抗菌药物耐药性均较高。尽早获取结膜囊分泌物进行细菌培养和药敏试验，对于临床诊断及合理选择抗生素治疗意义重大。

METHODS: Secretion specimens were taken from the patients with acute bacterial conjunctivitis in Xingtai Ophthalmic Hospital during January 2010 to December 2012 for bacterial culture. The pathogenic bacteria and drug sensitivity test were analyzed.

RESULTS: Of the 186 specimens, 150(80.6%)were positive in culture, in which 127(84.7%)were gram-positive bacteria, the first three species were Staphylococcus epidermidis(42/127, 33.1%), Human staphylococal(15/127, 11.8%), Staphylococcus haemolyticus(6/127, 4.7%). Twenty-three(15.3%)were gram-negative bacteria, the first four species were Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Enterobacter cloacae, the percentage of all resistance were 13.0%. Gram-positive strains were 100% sensitive to vancomycin, and were resistance to other antibiotics with different degree. Gram negative bacteria had the highest rate of drug resistance to cefoperazone 87.0%).

CONCLUSION:Some opportunistic bacteria including Staphylococcus epidermidis have become the majority of pathogenic bacteria of acute bacterial conjunctivitis in this area. Both gram-positive bacteria and gram-negative bacteria were higher to drug resistance. Early bacterial culture combined with drug sensitivity test is grate significance for clinical diagnosis and reasonable clinical chose of antibiotics.