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[摘要]
目的：探讨缺氧诱导因子-1α(hypoxia-inducible factor-1α，HIF-1α)及促红细胞生成素(erythropoietin，EPO)在碱烧伤大鼠角膜组织中的表达，评估其在角膜新生血管(corneal neovascularization，CNV)发生发展中的作用。
方法：健康3月龄SD大鼠，随机分为实验组和对照组，碱烧伤法建立CNV模型，分别于造模成功后1，3，5，7，14d测量CNV的长度并计算面积，同时取角膜组织以免疫组织化学检测HIF-1α及EPO的表达部位，并以RT-PCR法检测其mRNA的表达情况，实验数据采用SPSS 20.0处理。
结果：碱烧伤后1，3，5，7，14d，CNV的面积随时间逐渐增加，7d生长最为旺盛，14d后生长减慢。免疫组织化学提示：正常角膜各层未见HIF-1α的表达，可见微量EPO，碱烧伤后1d时HIF-1α及EPO免疫活性增强，主要表达于角膜上皮及内皮层。RT-PCR结果显示HIF-1α及EPO mRNA的表达在正常大鼠角膜组织中表达极少，在角膜碱烧伤后3d表达增强，7d达到高峰，14d后明显下降； 不同时间点组间比较存在统计学差异(P<0.05)。
结论：HIF-1α和EPO与CNV的形成密切相关。
[Key word]
[Abstract]
AIM: To describe the expression of hypoxia-inducible factor-1α(HIF-1α)and erythropoietin(EPO)in rats' corneal and evaluate its potential effect on corneal neovascularization(CNV)growth.
METHODS: The young SD rats(3mo)was chosen and randomly divided into 2 groups, which were experimental group and normal control group. CNV model was established by alkali burn, and the length and area of CNV was observed everyday after operation by slit lamp. After that, the expression of HIF-1α and EPO was measured by SABC and RT-PCR methods at 1, 3, 5, 7, and 14d after alkali burn. The data was analyzed by SPSS 20.0.
RESULTS: The area of CNV was increasing at 1, 3, 5, 7, and 14d after alkali burn, and the peak point appear at 7d. The growth speed was decreased after 14d. SABC method told us that no HIF-1α and very tiny amount EPO was detected at normal rats' corneal. The expression of the two factors increased at 1d after alkali burn in corneal epithelium and endoderm. The results of RT-PCR showed that a few amounts of HIF-1α and EPO mRNA were detected at normal group. The expression of the two factors was increased at 3d after alkali burn, and the peak value was found at 7d, however, it was decreased at 14d. Statistical difference was found at different time(P<0.05).
CONCLUSION: HIF-1α and EPO is closely related to CNV.
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