方法：将新鲜人眼球提取的HRCECs进行体外培养，最终用于实验的为生长良好的第3～4代细胞，实验分为空白对照组、低糖对照组、高糖对照组，高糖+不同浓度(50μL/mL，100μL/mL，200μL/mL)薏苡仁油组。不同浓度的薏苡仁油对体外培养的HRCECs增殖的抑制作用是通过用噻唑蓝比色法(MTT)检测。各分组HRCECs中VEGF的表达由免疫细胞化学法检测。

结果：MTT比色法结果显示：不同浓度的薏苡仁油作用于体外培养的HRCECs 48h，其细胞增殖抑制与高糖对照组相比有显著性差异(P<0.05)。48h内呈浓度依赖性。而低糖对照组与高糖对照组差异无统计学差异(P>0.05)。免疫细胞化学检测法表明：用50，100，200μL/mL的薏苡仁油作用于高糖下HRCECs 48h，高糖+不同浓度薏苡仁油组与高糖对照组相比VEGF表达下降明显(P<0.05)，将高糖+不同浓度薏苡仁油组之间进行两两比较亦具有统计学意义(P<0.05)。且呈浓度依赖性。高糖对照组与低糖对照组相比，VEGF表达明显(P<0.05)。

结论：薏苡仁油可抑制高糖环境下HRCECs的增殖和VEGF的表达。

METHODS: HRCECs extracted from human fresher eyeball and cultured in vitro, and ultimately used in the experiment were the growth of 3^rd～4^th cells, the experimental were divided into blank control group, low glucose control group, high glucose control group, high glucose +(50μL/mL, 100μL/mL, 200μL/mL)different concentrations Coix seed oil group. Detecting the multiplication of HRCECs by MTT, the immunocytochemical method was employed to detect the each group HRCECs of VEGF expression.

RESULTS:MTT assay results showed that: different concentrations of coix seed oil acted at HRCECs for 48h, inhibition of cell proliferation was significant difference compared with high glucose control group(P<0.05). Within 48h showed concentration dependence. There was no statistical difference between the low glucose group and high glucose control group(P>0.05). Immunocytochemical assay showed that: 50μL/mL, 100μL/mL, 200μL/mL Coix seed oil acted at HRCECs 48h, the expression of VEGF decreased significantly compared with the high glucose control group(P<0.05), and in a dose-dependent manner. However, in high glucose control group, the expression of VEGF was obvious higher than that of low glucose control group(P<0.05).

CONCLUSION:Coix seed oil can inhibit the HRCECs proliferation and suppress the VEGF expression in high glucose environment.