[关键词]
[摘要]
目的：探讨外源性过氧化物酶增殖激活受体-γ共激活子-1α(peroxisome-proliferator-activated receptor-γ coactivator-1α，PGC-1α)对人视网膜血管内皮细胞(human retinal vascular endothelial cell，HRVEC)中血管内皮生长因子(vascular endothelial growth factor，VEGF)表达的影响。
方法：HRVEC加入重组PGC-1α蛋白，未加入重组蛋白的细胞作为对照组。加入重组蛋白24h后，将两组细胞分别置于常氧(20% O2)和低氧(1% O2)环境下继续培养16h，采用real-time PCR、酶联免疫吸附试验(ELISA)及免疫荧光细胞化学法分别检测VEGF mRNA和蛋白的表达变化。
结果：real-time PCR，ELISA和免疫荧光细胞化学法检测发现，常氧和低氧环境下HRVEC加入重组PGC-1α蛋白后，VEGF mRNA和蛋白的表达均较对照组上调，差异有统计学意义(P<0.01)。
结论：PGC-1α在常氧和低氧环境中均能上调HRVEC中VEGF的表达。
[Key word]
[Abstract]
AIM: To investigate the effect of exogenous peroxisome-proliferator-activated receptor-γ coactivator-1α(PGC-1α)on vascular endothelial growth factor(VEGF)expression in human retinal vascular endothelial cells(HRVEC).
METHODS: Recombinant PGC-1α protein was added to HRVEC, and no recombinant PGC-1α protein was added to HRVEC as control group. After 24h of incubation, two groups of cells were then placed into a normoxic(20% O2)or hypoxic(1% O2)environment for another 16h. The expression of VEGF mRNA and protein were detected by real-time PCR, ELISA and immunofluorescence cytochemistry.
RESULTS: VEGF mRNA and protein levels in the cells were significantly increased by recombinant PGC-1α protein both under normoxia and hypoxia conditions as compared with control groups(P<0.01).
CONCLUSION: PGC-1α can upregulate the expression of VEGF in HRVEC under normoxia and hypoxia conditions.
[中图分类号]
[基金项目]
国家自然科学基金项目(No.81000387)
