方法：分离培养大鼠角膜基质细胞，根据PFD用药的不同浓度分为对照组(0mg/mL)、实验组Ⅰ(0.15mg/mL)、实验组Ⅱ(0.3mg/mL)、实验组Ⅲ(1mg/mL)，加药48h后应用CCK-8法检测其对角膜基质细胞增殖能力的影响，免疫细胞化学和Western-blot分别检测ki-67和TGF-β₁表达的变化。

结果：CCK-8结果显示，相比对照组，各实验组对角膜基质细胞的增殖均有明显的抑制作用，且随浓度的增大其抑制作用明显增强(均P<0.05)； 免疫细胞化学显示PFD能明显降低ki-67阳性指数(P<0.05)； Western-blot结果显示，PFD能降低TGF-β₁的表达，且呈剂量依赖关系(P<0.05)。

结论：PFD对大鼠角膜基质细胞的增殖具有明显抑制作用，抑制机制与下调TGF-β₁表达密切相关，在减轻角膜创伤愈合方面具有潜在的运用前景。

METHODS: Corneal stromal cells from 8 to 10wk SD rats were isolated, cultured and treated with different concentrations of PFD 0mg/mL(control group), 0.15mg/mL(experimental group Ⅰ), 0.3mg/mL(experimental group Ⅱ), 1mg/mL(experimental group Ⅲ)for 48h. CCK-8 assay was performed to assess cell proliferation, while immunocytochemistry and Western Blot were used to detect the expression of ki-67 and TGF-β₁ expression, respectively.

RESULTS: Compared with control group, PFD significantly inhibited the proliferation in a dose-dependent manner(all P<0.05), so was protein expression of ki-67. PFD significantly down-regulated the expression of TGF-β₁ in a dose-dependent manner(P<0.05).

CONCLUSION: Pirfenidone can significantly inhibit the proliferation of rat corneal stromal cell by down regulating TGF-β₁ expression, therefore, it has potential prospect in lightening the corneal wound healing reaction.