方法：选取40只SD大鼠行腹腔注射碘酸钠(NaIO₃，30g/L，100mg/kg)造视网膜变性模型，分为A组不干预组，B组BMSCs注射组，C组BMSCs+ChABC注射组，D组PBS注射组。造模后28d将ChABC处理或未处理的BMSCs注射入大鼠视网膜下腔，对照组注射PBS液，21d后处死大鼠并取出眼球，行视网膜HE染色、视网膜细胞凋亡及免疫组化检测。

结果：B组凋亡率、外核层细胞数与A组、D组比较，差异均有统计学意义(P<0.05)。C组凋亡率、外核层细胞数与A组、D组比较，差异均有统计学意义(P<0.05)。B组凋亡率、外核层细胞数与C组相比，差异无统计学意义(P>0.05)。免疫组化显示BMSCs在眼内表达GFAP抗原。

结论：BMSCs联合ChABC行视网膜下腔注射可缓解视网膜变性大鼠光感受器细胞的凋亡，延缓细胞数目的减少，从而保护视网膜光感受器细胞。

METHODS: Forty Sprague Dawley rats(SD rats)were intraperitoneally injected with NaIO₃ (30g/L, 100mg/kg)to establish the retinal degeneration models(postnatal 28d). These rats were devided into 4 groups. Group A was not injected, group B was injected with BMSCs, group C was injected with BMSCs and ChABC, and group D was injected with phosphate buffer saline(PBS). After 28d, subretinal injection were applied. Hematoxyln-eosinstaining(HE), tunel and immunohistochemistry were performed at 21d after subretinal injection.

RESULTS: Photoreceptor number and photoreceptor apoptosis rate of B and C groups were more than those of A and D groups, and there was significant difference statistically(P<0.05). Photoreceptor number and photoreceptor apoptosis rate of group B were compared with those of group C, and there was no statistical significance between B and C groups(P>0.05). Glial fibrillary acidic protein(GFAP)was expressed by BMSCs after intraocular injection.

CONCLUSION: BMSCs and ChABC injected into subretinal space may alleviate photoreceptor apoptosis so as to protect retinal photoreceptor cells in degenerated rats.