方法：性成熟的健康雌性SD大鼠40只，随机分为研究组、对照组，每组20只，研究组切除双侧卵巢、低浓度萘长间隔给药，建立围绝经期ARC模型； 对照组常规饲养。以消减杂交改良法克隆MRG15，并对其cDNA片段应用地高辛标记制作探针。获取两组大鼠晶状体前囊膜切片后，再通过原位杂交法明确晶状体上皮细胞的表达差异。

结果：克隆出的MRG15通过BamH1和EcoR1酶切以及琼脂糖电泳，可获取长为639bp的cDNA片段。GeneBank对比显示，其同源性达到99.0%。原位杂交显示，ARC患者及正常晶状体上皮细胞内均可见MRG15表达。研究组阳性细胞百分率与对照组相较，有统计学差异(P<0.05)。研究组积分指数与对照组相较，有统计学差异(P<0.05)。

结论：MRG15在ARC和正常晶状体上皮细胞中均有表达，并且ARC较正常晶状体上皮细胞表达增高，这可能与MRG15对晶状体上皮细胞中一部分关键基因的转录产生抑制效应有关，通过降低晶状体上皮细胞的功能，使其出现衰老样改变，进而形成白内障，临床应对此进行更进一步的研究。

METHODS: Forty mature healthy female SD rats were randomly divided into study group and control group, 20 rats in each group, the study group were ovariectomized, low concentration of naphthalene long interval of administration, the establishment of perimenopausal ARC model, the control group of conventional farming. In the subtractive hybridization cloning by MRG15, and make the probe of the cDNA fragment using digoxigenin labeled. Access to the two groups of rats anterior lens capsule after slicing, and then through the differential expression in situ hybridization clear lens epithelial cells.

RESULTS: The cloned MRG15 through BamH1, EcoR1 enzyme digestion and agarose gel electrophoresis, available for 639bp long cDNA fragment. GeneBank display contrast, their homology was 99.0%. In situ hybridization, ARC patients and normal lens epithelial cells were observed in the expression of MRG15. Study group the percentage of positive cells compared with control group, showed a significant difference(P<0.05). Integral index study group and the control group compared with, was significantly difference(P<0.05).

CONCLUSION: ARC, MRG15 in normal lens epithelial cells expressed ARC, and compared with the normal expression of lens epithelial cells, which may produce inhibitory effects are associated with MRG15 transcription in human lens epithelial cells in the part of key genes, by reducing the lens epithelial cell function, make its appear aging, and the formation of cataract, clinical response to this should further study.