方法：新西兰白兔32只随机分为四组：标准组、实验Ⅰ组、实验Ⅱ组和实验Ⅲ组，双眼均行标准小梁切除术。术中标准组于巩膜瓣下一次性应用0.3mg/mL丝裂霉素C棉片2min，其它3组术中分别于巩膜瓣下一次性应用0.3，0.4，0.5mg/mL苏拉明棉片2min。分别在术后第3，7，15，30d检查眼压、滤过泡情况，取滤过道附近组织分别行HE染色和免疫组化染色。

结果：术后第7，15，30d，四组眼压、功能性滤过泡、PCNA 染色阳性细胞核数比较，实验Ⅱ组和Ⅲ组与实验Ⅰ组和标准组比较，差异均有统计学意义(P<0.05)； 实验Ⅰ组与标准组的差异均无统计学意义(P>0.05)。实验Ⅲ组的眼压和PCNA 染色阳性细胞核数与实验Ⅱ组比较，差异均有统计学意义(P<0.05)； 实验Ⅲ组的功能性滤过泡与实验Ⅱ组比较，差异无统计学意义(P>0.05)。实验Ⅱ组和实验Ⅲ组的滤过道均清晰可见，滤过道开放程度均高于标准组和实验I组。

结论：苏拉明的浓度对其在兔小梁切除术中的作用效果有显著影响。0.3mg/mL苏拉明的作用效果与MMC相当； 0.4，0.5mg/mL苏拉明的作用效果显著优于MMC； 0.5mg/mL苏拉明的降眼压和抑制成纤维细胞增殖的效果优于0.4mg/mL苏拉明。

METHODS:Thirty-two albino rabbits were randomly divided into four groups, including standard control group, experimental group Ⅰ, experimental group Ⅱ, and experimental group Ⅲ. Each eye was performed standard trabeculectomy. During surgery, standard control group was given a piece of cotton with 0.3mg/mL mitomycin C(MMC)for 2min, and the other three groups were given a piece of cotton with 0.3, 0.4, and 0.5mg/mL suramin respectively for 2min. The filtering blebs and intraocular pressure(IOP)were observed at the 3, 7, 15, and 30d after surgery. Some conjunctiral specimen were observed with hitochemicall(HE staining)and immunohistochemicall methods.

RESULTS: At postoperative 7, 15, and 30d, the changes of the IOP, functional filtering blebs, and the number of positive cell nuclear in experimental group Ⅱ and experimental group Ⅲ were significantly different compared with those in standard control group and experimental group Ⅰ(all P<0.05), and the differences between experimental group Ⅰ and standard control group were not significant(P>0.05). The changes of the IOP and the number of positive cell nuclear in experimental group Ⅲ were significantly different compared with those in experimental group Ⅱ(P<0.05), whereas the differences in functional filtering blebs between experimental group Ⅲ and experimental group Ⅱ were not significant(P>0.05). The status of filtering channel in experimental group Ⅱ and experimental group Ⅲ were better than those in experimental group Ⅰ and standard control group.

CONCLUSION: The concentration of suramin has a significantly influence on its effect. When the concentration is 0.3mg/mL, the antiproliferative effect of suramin is no more than that of MMC. The effect of 0.4,0.5mg/mL suramin is better than MMC. 0.5mg/mL suramin has a better effect on controlling IOP and suppressing the growth of fibroblasts than 0.4mg/mL suramin.