方法：此前瞻性研究中纳入了原发性开角型青光眼患者11例11眼(平均年龄：62.7±6.1岁)，高眼压患者12例12眼(平均年龄：62.8±6.4岁)及健康人12例12眼(平均年龄：62.9±6.03岁)。这些患者均是最近被诊断出患有原发性开角型青光眼及高眼压，且之前未接受过抗青光眼方面的治疗。均行结膜印迹细胞学检查、泪膜破裂时间和基础泪液分泌试验。每组印迹细胞学检查的样本根据Nelson分级法分为0～3级。应用Kruskal-Wallis检验和Dunn多重比较检验进行统计分析。

结果：原发性开角型青光眼患者，高眼压患者及正常人群平均基础泪液分泌值分别为10.4±1.3，10.9±1.2和11.1±1.1 mm/5min，其差距没有统计学意义(P=0.33)； 三组的泪膜破裂时间分别为11.2±1.1， 11.3±1.1和11.8±1.2s，其差距没有统计学意义(P=0.35)。原发性开角型青光眼患者中6眼(54.5%)为0级， 5眼(45.5%)为1级。高眼压患者中6眼(50%)为0级， 6眼(50%)为1级，健康人中6眼(50%)为0级， 6只眼(50%)为1级(P=0.97)。

结论：氧化应激可能会导致青光眼，眼表疾病，泪腺功能障碍及机体杯状细胞所分泌的黏液减少。原发性开角型青光眼患者，高眼压症患者及健康人群间的印象细胞学检查数值并无显著差异。

METHODS: Eleven eyes of 11 patients with POAG(mean age: 62.7±6.1y), 12 eyes of 12 patients(mean age: 62.8±6.4y)with OHT and 12 eyes of 12 normal subjects(mean age: 62.9±6.03y)were included to this prospective study. The patients with POAG and OHT had been recently diagnosed with these diseases and none of them had taken anti-glaucoma treatment before. In addition to conjunctival impression cytology, tear break-up time(TBUT)and basal Schirmer's tests(BST)were performed. Impression cytology specimens of each group were graded and scored in the range of 0-3 according to Nelson's method. Kruskal-Wallis analysis and Dunn's multiple comparison tests were used for statistical analysis.

RESULTS: The mean BST values were 10.4±1.3, 10.9±1.2 and 11.1±1.1 mm/5min of POAG, OHT and control groups respectively. The differences among the BST values of the POAG, OHT and control group were not statistically significant(P=0.33). The mean TBUT values were 11.2±1.1, 11.3±1.1 and 11.8±1.2s in POAG, OHT and normal subjects respectively. The differences among the BUT values of the POAG, OHT and control group were not statistically significant(P=0.35). Six eyes(54.5%)revealed grade 0 and 5 eyes(45.5%)revealed grade 1 impression cytology scores in POAG group. Six eyes(50%)revealed grade 0 and 6 eyes(50%)revealed grade 1 impression cytology scores in OHT group and 6 eyes(50%)revealed grade 0 and 6 eyes(50%)revealed grade 1 impression cytology scores in normal subjects(P=0.97).

CONCLUSION: Oxidative stress may cause glaucoma, ocular surface diseases, lacrimal gland malfunction and a decrease in mucus secretion ofgoblet cells in all of the body. There were no significant differences between the impression cytology scores of patients with POAG, OHT and normal subjects.