方法：选取2012-01/2014-10来我院治疗的糖尿病性白内障患者、年龄相关性白内障患者和外伤性白内障患者各20例，采用RT-PCR法检测各组患者晶状体上皮细胞中SIRT1基因含量，Western blot法检测晶状体上皮细胞中SIRT1蛋白含量，TUNEL法检测晶状体上皮细胞的凋亡率。

结果：RT-PCR检测结果显示，外伤性白内障患者组SIRT1 mRNA相对含量最高为1.000±0.078，其次为年龄相关性白内障患者组为0.427±0.067，糖尿病性白内障组为0.389±0.112，与外伤性白内障组比较，差异有统计学意义(P<0.05)； Western blot检测显示，外伤性白内障患者晶状体上皮细胞中SIRT1 蛋白的表达量最高，其次是年龄相关性白内障组，糖尿病性白内障组SIRT1蛋白的表达量最低； TUNEL法检测结果显示，外伤性白内障组和年龄相关性白内障组患者LECs细胞凋亡率分别为(4.5±2.3)%和(8.7±4.1)%，差异无统计学意义； 而糖尿病性白内障组LECs凋亡率为(24.3±6.1)%，与外伤性白内障组及年龄相关性白内障组比较差异有统计学意义(P<0.05)。

结论：糖尿病性白内障患者晶体上皮细胞中SIRT1基因及蛋白表达下降，提示该基因参与了糖尿病性白内障的发生，这为我们今后进一步的研究提供了可靠的理论依据。探索调节SIRT1基因在晶状体上皮细胞中表达的有效途径，将为糖尿病性白内障的早期干预治疗提供新的思路。

METHODS:Twenty diabetic cataract patients, 20 age-related cataract patients and 20 traumatic cataract patients diagnosed from January 2012 to October 2014 in our hospital were selected. RT-PCR method was used to detect the content of SIRT1 gene in LECs of each group patients. Western blot method was used for the detection of SIRT1 protein content in lens epithelial cells, apoptosis rate of LECs was detected by TUNEL.

RESULTS: RT-PCR reaults showed that the relative content of SIRT1 mRNA in patients of traumatic cataract group was highest for 1.000±0.078, followed by the age related cataract group was 0.427±0.067, then diabetic cataract group was 0.389±0.112, those two groups compared with the traumatic cataract group, the difference was statistically significant(P<0.05); Western blot showed that SIRT1 protein expression in LECs of traumatic cataract patients was the highest, followed by the age related cataract group, diabetic cataract group the expression of SIRT1 protein was the minimum. The results of TUNEL showed that apoptosis rate of traumatic cataract group and age group LECs rates were(4.5±2.3)% and(8.7±4.1)%, respectively, the difference was not statistically significant; while the diabetic cataract group of LEC apoptosis rate was(24.3±6.1)%, by comparing traumatic cataract group and age related cataract group, the difference was statistical significance(P<0.05).

CONCLUSION: Expression of SIRT1 gene and protein decreased in LECs of diabetic cataract patients, suggesting that this gene was involved in diabetic cataract, this provides reliable theoretical basis for our further research in the future. Regulation of SIRT1 gene expression in LECs will explore the effective ways and provide a new idea for the diabetic cataract intervention treatment.