方法：收集青光眼患者41例46眼，分别于小梁切除术前1d，术后1d； 1wk； 1mo行第一次泪膜破裂时间(first tear break up time，FTBUT)、平均泪膜破裂时间(average tear break up time，ATBUT)、非侵入式泪河高度(non-invasive tear meniscus height，NITMH)、脂质层厚度分级及睑板腺完整度评分检查。

结果：与术前1d相比，术后1d； 1wk； 1mo的FTBUT、ATBUT缩短(P<0.05)，脂质层变薄(P<0.017)。术后1d的NITMH高于术前1d，而术后1wk和1mo的NITMH低于术前1d(P<0.05)。睑板腺完整度评分手术前后差异无统计学意义(P>0.05)。

结论：Keratograph 5M眼表综合分析仪可以快速、精确地评估泪膜的功能。青光眼小梁切除术在术后短期内可对泪膜的稳定性及泪液的分泌量产生不良影响。

METHODS: Fourty-one patients(46 eyes)of glaucoma who underwent trabeculectomy were involved. First tear break up time(FTBUT), average tear break up time(ATBUT), non-invasive tear meniscus height(NITMH), lipid layer thickness and meibomian gland scores were measured at 1d preoperatively and at 1d, 1wk, 1mo postoperatively.

RESULTS:At 1d, 1wk and 1mo postoperatively, FTBUT and ATBUT decreased greatly(P<0.05), as well as lipid layer thickness(P<0.017). NITMH at 1d postoperatively increased significantly, but at 1wk and 1mo postoperatively decreased greatly(P<0.05). There was no significantly difference in meibomian gland scores between preoperation and postoperation(P>0.05).

CONCLUSION:Keratograph 5M could be used to evaluate tear film function rapidly and accurately. Trabeculectomy significantly alters tear film stability and tear secretion in the short term after operation.