方法：首先，将不同浓度(200、400、600、800nmol/L)TSA作用HTFs共24h后，MTT法检测其对细胞活力的影响； 然后，不同浓度(400、600nmol/L)TSA与5ng/mL TGF-β₁混合作用于HTFs共24h，MTT法检测其对细胞活力的影响； 最后，RT-PCR和Western-blot法分别检测不同浓度(400、600nmol/L)TSA与5ng/mL TGF-β₁混合以及600nmol/L TSA对HTFsⅠ型胶原纤维的mRNA及蛋白表达的影响。

结果：MTT证实，与对照组相比，400nmol/L及以上浓度TSA作用组，HTFs活力显著下降(P<0.05)； 两种浓度(400、600nmol/L)TSA均可减弱TGF-β₁促HTFs增殖的作用(P<0.05)； RT-PCR和Western-blot证实两种浓度(400、600nmol/L)TSA对TGF-β₁诱导上调的Ⅰ型胶原纤维在基因转录及蛋白表达水平有逆转作用。

结论：TSA能够抑制TGF-β₁诱导的HTFs增殖，并减弱Ⅰ型胶原纤维基因转录及蛋白表达水平。

METHODS: Firstly, the effect of TSA at different concentrations(200, 400, 600 and 800nmol/L)on HTFs viability after 24h was detected using MTT proliferation assays. Then, the effect of TSA at 400nmol/L and 600nmol/L mixed with 5ng/mL TGF-β₁ on HTFs viability after 24h were investigated using MTT proliferation assays. Furthermore, the mRNA and protein expression of collagen Ⅰ in HTFs after treatment with TSA at different concentrations(400, 600nmol/L)mixed with 5ng/mL TGF-β₁ as well as 600nmol/L TSA were detected by RT-PCR and Western-blot.

RESULTS: Compared with control group, the results of MTT showed that HTFs viability decreased significantly after treated with TSA at 400, 600 and 800nmol/L(P<0.05). The HTFs proliferation induced by TGF-β₁ could be attenuated by TSA at 400 and 600nmol/L(P<0.05). The results of RT-PCR and Western-blot confirmed that TSA at 400 and 600nmol/L had reversal effect on up-regulated gene transcription and protein expression levels of collagen Ⅰ induced TGF-β₁.

CONCLUSION:TSA can inhibit the HTFs proliferation induced by TGF-β₁ and attenuate gene transcription and protein expression of collagen Ⅰ.