方法：采用临床病例对照研究，以2012-01/2013-12期间就诊的90例糖尿病患者为研究对象，分为糖尿病无视网膜病变(no diabetic retinopathy，NDR)组、单纯型糖尿病视网膜病变(simple diabetic retinopathy，SDR)组和增殖型糖尿病视网膜病变(proliferative diabetic retmopathy，PDR)组三组，以健康志愿者(normal controls，NC)为对照组，采用双抗体夹心法酶联免疫吸附法检测患者血清中Chemerin水平，同时检测TNF-α、脂联素等炎症因子水平，收集患者体重指数、血脂、基础疾病等临床资料。

结果：NC组、NDR组、SDR组和PDR组患者血清Chemerin依次为12.35±3.56、26.50±5.34、34.67±7.72、 40.97±8.97μg/L，SDR组、PDR血清Chemerin水平明显高于NC组和NDR组，差异有统计学意义(P<0.01)，血清Chemerin水平与TNF-α正相关(r=0.8746，P<0.01)，与脂联素负相关(r=-0.7418，P<0.01)。Logistic多元回归分析结果显示，Chemerin为DR独立危险因素之一(OR=8.458，95%CI：1.611～44.410，P=0.012)。

结论：DR患者血清中Chemerin水平升高，其可能参与了DR的发生发展过程。

METHODS: A total of 90 diabetes patients from January 2012 to December 2013 were enrolled, including non-diabetic retinopathy(NDR,n=30), simple diabetic retinopathy(SDR, n=30), proliferative diabetic retinopathy(PDR, n=30). Thirty healthy persons were included as normal control(NC). Chemerin level, tumor necrosis factor-α(TNF-α), adiponectin were assessed by enzyme-linked immunosorbent assay.Clinical characters including BMI, underlying diseases, blood lipids ect. were collected.

RESULTS: The serum levels of Chemerin in NC, NDR, SDR, and PDR group were 12.35±3.56, 26.50±5.34, 34.67±7.72, 40.97±8.97μg/L respectively. The serum levels of Chemerin in SDR and PDR group were significantly higher than those in NC and NDR group(P<0.01). Serum levels of Chemerin positively correlated with TNF-α(r=0.8746, P<0.01)and negatively correlated with adiponectin(r=-0.7418,P<0.01). The results of Logistic regression analysis showed that Chemerin was an independent risk factor for DR(OR=8.458, 95%CI: 1.611～44.410, P=0.012).

CONCLUSION: The results show that serum Chemerin level is significantly elevated in DR patients, and Chemerin may be involved in the development of DR.