方法：SD大鼠36只随机分为对照组(A组，n=12)和造模组(n=24)，造模组注射链脲佐菌素(STZ)60mg/kg，造模成功后随即分为糖尿病组(B组，n=12)、复方血栓通干预组\〖C组，1g/(kg·d)，n=12\〗，于实验第6、12wk分别在各组处死6只大鼠，RT-PCR方法检测视网膜组织中CTGF mRNA水平。

结果：与A组相比，B组、C组大鼠视网膜CTGF mRNA于第6、12wk表达均增加(均P<0.05)，C组视网膜组织CTGF mRNA表达于第6、12wk均较B组明显下调(P<0.05)； 电镜显示B组大鼠视网膜毛细血管内皮细胞肿胀，胞质内线粒体肿胀，吞饮小泡增多，毛细血管基底膜电子密度不均匀，有结节样增厚现象，C组视网膜病变较B组减轻。

结论：CTGF可能参与糖尿病视网膜病变发生，复方血栓通对糖尿病大鼠视网膜具有保护作用，可能通过下调CTGF mRNA产生作用。

METHODS:Sprague-Dawley(SD)rats were divided into normal group(group a,n=12)and model group(n=24). Rats in model group received intraperitoneal injection of streptozotocin(60mg/kg body weight)to get diabetic rats,which were randomly subdivided into diabetic group(group B,n=12)and xueshuantong intervention group \〖group C,1g/(kg·d),n=12\〗. At 6th and 12th wk of the experiment, 6 rats randomly selected from every group,were executed to get retinal tissue for detecting CTGF-mRNA by electron microscope.

RESULTS:Compared with group A,the content of CTGF-mRNA in retina tissue increased at 6, 12wk in both group B and C(P<0.05). Compared with group B,the content of CTGF-mRNA in retina tissue in group C decreased at 6, 12wk(P<0.05). Electron microscope showed:in group B,the capillary endothelial cells and mitochondria became swollen; the amount of pinocytosis vesicle increased; the electron density in capillary basement membrane was not homogeneous and nodular thickening phenomenon appeared; compared with group B,the changes in group C were alleviated.

CONCLUSION:CTGF may be involved in the progress of DR in diabetic rats. Compound xueshuantong may exert its protective effect through decreasing the expression of CTGF in the retina of diabetic rats.