[关键词]
[摘要]
目的：观察骨形态发生蛋白-6(bone morphogenetic protein，BMP-6)对过氧化氢(hydrogen peroxide，H
2O
2)作用的人视网膜色素上皮株ARPE-19细胞的细胞形态、增殖以及凋亡的影响。
方法：常规培养ARPE-19细胞，分为正常对照组、75μmol/L H2O2及150ng/mL BMP-6、75μmol/L H2O2+150ng/mL BMP-6环境中培养3、6、9、12h后，MTT比色法检测细胞的活性； 流式细胞仪检测细胞的周期及凋亡变化。
结果：H2O2作用组的细胞活性随着作用时间的延长逐步降低； 而BMP-6+H2O2组的细胞活性则较H2O2组高，在3h及6h时两组相比差异有统计学意义(P<0.05)； 细胞形态观察发现：H2O2培养6h后，细胞数量减少，细胞发生脱落，而添加BMP-6后细胞脱落及凋亡均要明显减少。
结论：BMP-6可以一定程度上保护视网膜色素上皮受到氧化应激的损伤。
[Key word]
[Abstract]
AIM:To investigate the effect of bone morphogenetic protein 6(BMP-6)on cellular morphology, proliferation and apoptosis of retinal pigment epithelial cells(ARPE-19)incubated in hydrogen peroxide(H
2O
2).
METHODS:ARPE-19 cells were cultured conventionally and divided into four groups. One group was untreated as blank group, the other three groups were incubated in 75μm/L H2O2, 150ng/mLBMP-6 or75μm/L H2O2+150ng/mL BMP-6. All the groups were incubated for 3h, 6h, 9h and 12h. We tested the cell viabilitity by MTT. We used flow cytometry to test the cell cycle and cell apoptosis.
RESULTS:H2O2 significantly decreased the cell activity in time-dependent manner. The activity of cells with BMP-6+H2O2 was higher H2O2 group, and the differences between the two groups at 3h and 6h were significant(P<0.05). The observation on cellular morphology showed that the cell number decreased and the cell detachment after 6h incubated with H2O2, while the cells with BMP-6 were less cell detachment and apoptosis.
CONCLUSION:BMP-6 has protective effects on RPE cells from oxidative stress in certain extent.
[中图分类号]
[基金项目]
中国陕西省科学技术研究发展计划项目(No.2012K16-11-01)
