方法：将15只野生型(WT)小鼠作为对照组，10只Eaf2基因敲除(Eaf2 KO)小鼠作为实验组。两组均取14周龄左右的小鼠作为研究对象。进一步分组为：WT-nonUV、WT-UV、Eaf2 KO-nonUV、Eaf2 KO-UV，共4组。使用裂隙灯显微镜观察小鼠白内障程度，利用晶状体混浊分类系统Ⅱ(LOCSⅡ)对小鼠白内障进行分级。然后断颈处死小鼠，摘取晶状体进行暗视野显微镜照相，利用Image J软件对晶状体混浊程度进行分析，并将各测量结果进行统计学处理。

结果：裂隙灯显微镜和暗视野显微镜的结果一致：WT-UV组及Eaf2 KO-UV组晶状体混浊程度明显高于WT-nonUV组及Eaf2 KO-nonUV组，其中WT-UV组明显高于Eaf2 KO-UV组，均具有统计学差异(P<0.05)。

结论：紫外线辐射能够导致小鼠白内障的形成，Eaf2蛋白质具有促进紫外线所致的小鼠白内障形成的作用。

METHODS:Fifteen wild type mice were used as the control group, and 10 Eaf2 KO mice were used as the experimental group. The 14-week mice were taken as the research objects in the two groups. So the subgroups were: WT -nonUV, WT -UV, Eaf2 KO-nonUV and Eaf2 KO-UV, a total of 4 groups. Observe the lens of mice in vivo with slit lamp microscope, grade the lens opacity with Lens Opacities Classification System II(LOCSII). Then the mice were sacrificed by breaking the neck, the lens were removed and were observed by dark field microscopy. According to the captured images, the proportion of cataract region was analyzed using Image J software. The data of the two groups were statistically analyzed.

RESULTS: The results detected by the two methods were similar. In WT-UV group and Eaf2 KO-UV group, the degree of lens opacity was significantly higher than those of WT-nonUV group and Eaf2 KO-nonUV group. The lens opacity of WT-UV group was significantly higher than that in Eaf2 KO-UV group, and the difference was statistically significant(P<0.05).

CONCLUSION: Ultraviolet radiation can lead to the formation of cataract in mice. Eaf2 protein can promote the formation of cataract in mice caused by ultraviolet.