方法：将24只家兔按照随机等分的方法分为3组，即姜黄素干预青光眼模型组(姜黄素组)、未给予姜黄素干预的青光眼模型组(青光眼组)和不经任何干预措施的正常对照组(正常组)。应用前房灌注升高眼压的方法给姜黄素组和青光眼组的家兔建立急性高眼压模型，急性高眼压模型造模成功后，给予姜黄素组家兔玻璃体腔注射比例浓度为0.1mg/0.1mL的姜黄素，青光眼组家兔玻璃体腔注射相同体积的生理盐水，每天注射1次，共7d。正常对照组不做上述任何处理直接取眼球。实验完成后，通过免疫组织化学法检测三组家兔眼球视网膜神经节细胞Thy-1的表达并计数。

结果：姜黄素组和正常组的视网膜神经节细胞Thy-1表达之间差异无统计学意义(P>0.05)； 青光眼组和正常组的视网膜神经节细胞Thy-1表达差异有显著统计学意义(P<0.01)。姜黄素组、正常组和青光眼组的视网膜神经节细胞密度分别为20.3±2.7、21.5±1.8和15.1±2.3个/高倍视野。

结论：在姜黄素作用的急性高眼压模型家兔的实验中，姜黄素可以通过提高视网膜神经节细胞的Thy-1的表达，表明其在一定程度上能够降低急性高眼压状态下家兔视网膜神经节细胞的损伤，推测姜黄素可能对视网膜在特定情况下具有一定的保护作用。

METHODS: Twenty-four adult rabbits were divided randomly into normal control group, model group and curcumin group. Except normal control group, the rabbit model with acute ocular hypertension was established by increasing intraocular pressure through anterior chamber infusion. After the establishment of the model, rabbits in curcumin group were injected intravitreally with curcumin(0.1mg/0.1mL)for 7d. Rabbits in mdodel group were injected with physiological saline replaced of the same volume curcumin. The oculars in normal control group without any treatments were acquired directly. The levels of Thy-1 in RGCs were detected by immunohistochemistry. The density of RGCs was counted too.

RESULTS: Thy-1 expressed in the RGCs in normal control group and curcumin group had no significant difference(P>0.05).While the difference between model group and normal control group on Thy-1 expression was statistically significant(P<0.05). The density of RGCs in curcumin group, normal control group and model group were 20.3±2.7, 21.5±1.8 and 15.1±2.3 cells/HP.

CONCLUSION: In this study on curcumin in adult rabbits with acute ocular hypertension, curcumin can increase the expression of Thy-1, which shows that it can partly reduce the injury of RGCs in rabbits with acute intraocular hypertension and curcumin may be able to protect the retina under certain situation.