方法：将SD大鼠60只随机分为糖尿病组和对照组。采用STZ腹腔注射制作糖尿病大鼠模型，糖尿病组大鼠腹腔注射STZ 60mg/kg，对照组腹腔注射同等剂量的生理盐水。分别于1、2和4mo时处死大鼠，摘取视网膜，HE染色观察视网膜结构变化，荧光血管造影观察视网膜血管变化，TUNEL染色观察视网膜细胞凋亡情况，Western Blot检测视网膜中HMGB1和NF-κB的表达。

结果：与对照组相比，糖尿病组大鼠视网膜各层细胞排列紊乱，细胞密度减低，可见微血管病变，以及内、外核层变薄和神经节细胞的凋亡； 荧光血管造影见周边毛细血管迂曲，可见血管闭塞及无灌注区； Western Blot检测HMGB1和NF-κB表达呈时间依赖性增高，且两者表达呈正相关(P<0.05)。

结论：HMGB1在糖尿病大鼠视网膜中表达增加，HMGB1可能通过NF-κB信号通路参与了糖尿病视网膜病变的发生。

METHODS:Sixty SD rats were randomly divided into diabetic group and control group. Diabetic rat model was produced by intraperitioneal injection of 1% STZ with 60mg/Kg weight. The rats in control group received intraperitioneal injection of normal saline with same dosage. After injection, the rats were sacrificed and eyeballs were enucleated for HE staining, the retina fluorescence angiography, TUNEL and Western Blot detection at 1, 2 and 4mo for the expressions of HMGB1 and NF-κB.

RESULTS:Compared with the control group, the retinal cells disorder, cell densities decreases, microvasculars occlusion were founded with inner and outer nuclear layer thinning and ganglion cell apoptosis. The fluorescence angiography showed that peripheral capillaries became circuitous and vascular occlusion and non-perfusion area could be seen. The expressions of HMGB1 and NF-κB were higher than those of control with time dependence and they had significant positive correlations(P<0.05).

CONCLUSION:The expression of HMGB1 increases in diabetic rat retina, which may involve in the occurrence of diabetic retinopathy through the NF- κB pathway.