方法：通过双侧睾丸切除去势手术，改变体内雄激素水平，观察泪膜破裂时间的变化，同时在不同时间点采用RT-PCR法与Western-Blot法分别检测小鼠角膜上皮细胞中Muc1和Muc4的mRNA和蛋白水平的表达变化。

结果：去势1wk后小鼠睾酮质量浓度下降至0ng/μL； 正常对照组、伪手术组及去势术后1、2、4、6、8wk组小鼠BUT分别为68.33±12.86、62.47±3.75、58.67±10.03、47.17±7.64、39.51±3.39、32.67±3.88、31.00±2.36s，其中术后2、4、6、8wk组小鼠泪液BUT值较正常对照与伪手术组均明显缩短，差异均有统计学意义(均P<0.05)。Muc1与Muc4的mRNA与蛋白水平表达，随雄激素浓度下降，表达下调(P<0.05)，Muc1在去势后第2wk表达最低，而Muc4在去势后第1wk表达降至最低。

结论：在体内，雄激素可调控小鼠角膜上皮Mucins表达，并缩短泪膜破裂时间，参与泪膜稳定性的维持。

METHODS: With orchiectomy operation, we set up mice model. And serum androgen concentration of mice was detected by radioimmunoassay. Break-up time(BUT)of tear film was tested in the different experimental points. Mice corneal epithelia were peeled and MUC1 and MUC4 mRNA and protein levels were measured by RT-PCR and Western blot.

RESULTS: The serum androgen concentration reduced to 0ng/μL at 1wk after orchiectomy. The BUT values were 68.33±12.86s, 62.47±3.75s, 58.67±10.03s, 47.17±7.64s, 39.51±3.39s, 32.67±3.88s and 31.00±2.36s in the normal control group, sham group and in orchectomy group at 1, 2, 4, 6 and 8wk, respectively, and the BUTs were significantly shorter in the orchectomy at 2, 4, 6 and 8wk groups than those in the normal control group(all at P<0.05). MUC1 and MUC4 mRNA and proteins levels decreased with androgen level lowering(P<0.05). Mucin1 level was the lowest at 2wk after orchiectomy, and the lowest Mucin4 level was found at 1wk after orchiectomy.

CONCLUSION:In vivo, androgen regulates Mucins expressions in mice corneal epithelial cells, makes BUT shorter,and influence the stability of tear film.