[关键词]
[摘要]
目的：探讨银杏内酯B对高糖诱导的人晶状体上皮细胞(human lens epithelial cells，HLECs)凋亡的影响。
方法：建立高糖诱导的HLECs模型，用不同浓度的银杏内酯B干预，MTT比色法检测细胞存活率，Hochest33258染色观察凋亡细胞形态，透射电镜观察细胞内超微结构变化，比色法检测凋亡相关因子Caspase-3及Caspase-9的表达。
结果：高浓度葡萄糖可抑制HLECs活性，诱导HLECs发生凋亡，引起细胞内Caspase-3及Caspase-9表达水平升高； 银杏内酯B能明显抑制高糖诱导的HLECs活力的下降，降低细胞凋亡率，减少高糖所致HLECs细胞内Caspase-3及Caspase-9的表达。
结论：银杏内酯B可能通过抑制Caspase-3及Caspase-9的表达而有效抑制了高糖诱导的HLECs凋亡。
[Key word]
[Abstract]
AIM: To study the effect of ginkgolide B on high glucose-induced human lens epithelial cell(HLEC)apoptosis.
METHODS: The high glucose-induced HLEC model was established. Different concentrations of ginkgolide B were intervened. Cell viability was assayed by MTT assay, morphology of cell apoptosis was observed by hochest33258 staining. Cell ultrastructural changes were detected by transmission electron microscopy. Expressions of apoptosis-related factors caspses-3 and caspase-9 were tested by colorimetric detection.
RESULTS: High glucose inhibited the activity of HLECs, induced apoptosis reaction of HLECs, caused high expression of apoptosis factors in cells; while ginkgolide B inhibited the decrease of cell viability induced by high glucose, decreased the HLEC apoptosis and reduced the expression of apoptosis factors Caspase-3 and Caspase-9.
CONCLUSION: Ginkgolide B may inhibit the expression of caspses-3 and caspase-9 then effectively inhibited high glucose-induced apoptosis in HLECs.
[中图分类号]
[基金项目]
国家自然科学基金(No.30973275)
