方法：收集27例角膜标本，年龄范围从0.2岁到78岁，所有标本经HE染色后行组织切片并通过400倍光学显微镜行显微照相，于每张相片中选取四个测量点并人工标记后弹力层的边界，通过软件Motic Images Plus 2.0自动计算其厚度并取平均值。采用线性回归方法分析角膜后弹力层厚度与年龄的关系。

结果：所有标本的角膜后弹力层厚度为1.78μm到9.30μm，平均4.63±2.00μm。角膜后弹力层厚度与年龄具有高正相关性(r=0.776， P=0.000)，线性回归方程可描述为：角膜后弹力层厚度(μm)=2.010+0.063年龄(岁)。

结论：中国人的角膜后弹力层厚度与年龄之间具有显著正相关性。

METHODS: Twenty-seven normal corneas with ages ranging from 0.2 to 78y old were collected. All specimens were stained with Hematoxylin and Eosin, and viewed and photographed at ×400 through a calibrated Motic light photomicroscope. Four measurement sites on each specimen micrograph were chosen and the boundary of Descemet's membrane was designated manually, then the Descemet's membrane thickness was measured by the software of Motic Images Plus 2.0 automatically. The relationship between Descemet's membrane thickness and age was analyzed by using linear regression methods.

RESULTS: The thickness of Chinese Descemet's membrane various from 1.78 to 9.30 μm, averaged 4.63±2.00 μm. There is a highly significant positive correlation between age and thickness of Descemet's membrane(r=0.776, P=0.000). The estimated equation that best describes the relationship of thickness with age can be expressed as: Thickness(μm)= 2.010+0.063y.

CONCLUSION: There is a significant positive association between age and the thickness of Descemet's membrane in Chinese people.