方法：采用聚合酶链反应联合限制性片段长度多态性分析(polymerase chain reaction-restriction fragment polymorphisms assay，PCR-RFLP)技术及基因测序技术，在大理白族人群中对150例T2DM患者及100例健康对照者(CON组)的IL-6-572C/G位点基因多态性进行检测。150例T2DM患者中，NDR(未合并DR)组57例，NPDR(合并非增生性糖尿病视网膜病变)组77例，PDR(合并增生性糖尿病视网膜病变)组16例。比较各组间基因型及等位基因分布频率，同时收集临床生化指标，最终数据使用SPSS22.0统计软件进行统计学分析。

结果：IL-6基因-572C/G位点组间基因型及等位基因频率相比较，差异均具有统计学意义(P<0.05)； 携带C等位基因的个体避免患T2DM的风险为G等位基因的1.182倍(95% CI：1.059～1.319，P=0.004)； 携带G等位基因的T2DM患者并发DR的风险为C等位基因的1.667倍(95% CI：1.195～2.326，P=0.003)，但基因型及等位基因频率在PDR组与NPDR组比较，差异均无统计学意义(P>0.05)； T2DM、NPDR+PDR组与CON组在空腹血糖、甘油三酯、体质量指数比较均有统计学差异(P<0.05)； NPDR组与PDR组相比只有空腹血糖比较差异有统计学意义(P<0.05)； 合并高血压的个体患T2DM的风险为未患高血压的 3.730倍(95% CI：2.060～6.754，P=0.000)，同时患DR的风险为3.997倍(95% CI：2.099～7.612，P=0.000)； 不同基因型间临床生化指标比较均无统计学差异(P>0.05)。

结论：IL-6基因-572C/G基因多态性与大理白族T2DM及DR的易感性相关，但临床生化指标对此易感关联没有协同作用，G等位基因是DR和T2DM发病的危险因素，但在NPDR进展至PDR过程中无意义，C等位基因对于T2DM和DR的发病具有保护作用； 高血压、空腹血糖、甘油三酯、体质量指数促进T2DM和DR的发病，空腹血糖在DR病情进展中具有重要作用。

METHODS: The polymorphisms of IL-6 gene -572C/G was analyzed by polymerase chain reaction-restriction fragment polymorphisms assay(PCR-RFLP)in 150 patients with T2DM \〖NDR(without DR)group 57 cases, NPDR(with non-proliferative diabetic retinopathy)group 77 cases, PDR(with proliferative diabetic retinopathy)group 16 cases\〗 and 100 healthy control group in Dali Bai population. The genotypes frequency, allele frequency of IL-6-572C/G gene and clinical data were compared between groups. The statistics software SPASS22.0 was used for statistical analysis.

RESULTS: The genotype and allele frequencies of IL-6 gene -572C/G between groups were compared statistically significantly(P<0.05); the incidence of T2DM in healthy control subjects with C allele significantly decreased in comparison with the carriers of G allele(OR=1.182, 95% CI: 1.059-1.319, P=0.004); the incidence of DR in T2DM with G allele significantly increased in comparison with the carriers of C allele(OR=1.667, 95% CI: 1.195-2.326, P=0.003), but there was no statistical difference between PDR group with NPDR group for -572C/G polymorphism(P>0.05). There was statistical significant between T2DM group, NPDR + PDR and control groups in fasting blood glucose, triglycerides, body mass index(P<0.05), PDR group was compared with NPDR group only in difference between fasting plasma glucose(P<0.05). The hypertension in individuals suffering from T2DM increased in comparison with not combing hypertension(OR=3.730, 95% CI: 2.060-6.754, P=0.000), and suffering from DR increased obviously(OR=3.997, 95%CI:2.099-7.612, P=0.000). The clinical data showed no significant difference(P>0.05)among different genotypes.

CONCLUSION: These results suggest that the -572C/G polymorphism in the promoter of IL-6 gene is associated with T2DM and DR in Dali Bai population, but the clinical data is not coordinative risk factor. G allele is a risk factor in the pathogenesis of T2DM and DR, but not for the progress in NPDR to PDR process, C alleles is a protective factor T2DM and DR. Hypertension, fasting blood glucose, triglycerides, body mass index were risk factors for T2DM and DR, fasting blood glucose condition in DR progress has an important role.