方法：对随访资料完整的35例70眼配戴3a夜用角膜塑形镜的青少年近视眼患者和同期配戴框架眼镜的15例30眼患者，行平均泪膜破裂时间、基础泪液分泌量、角膜曲率、角膜厚度、角膜内皮细胞检测。分析两组患者在泪膜稳定性及角膜生物学特性方面的差异。

结果：与框架镜组相比，患者在戴角膜塑形镜前后平均泪膜破裂时间差值、基础泪液分泌量差值、角膜厚度差值均无统计学意义(P=0.0922、0.0891、0.4181)； 角膜曲率差值、角膜内皮细胞变异系数差值、六角形细胞比例变化差值均有统计学差异(P=0.0000、0.0005、0.0002)。所有患者在随访期间均无严重并发症发生。

结论：长期夜戴角膜塑形镜对泪膜稳定性影响轻微，但会增加角膜内皮细胞的变异系数，降低六角形细胞的比例。为保证其使用的安全性，需密切随访观察，定期护理。

METHODS: Thirty-five myopia patients(70 eyes)worn overnight orthokeratology and fifteen myopia patients(30 eyes)worn frame glasses were involved in our study with complete follow-up information. We measured average tear breakup time(aBUT), basal tear secretion, corneal thickness and corneal curvature and corneal endothelium, then analyzed the differences in tear film stability and corneal biological characteristics between myopia patients of the both groups.

RESULTS: Compared with the frame glasses group, there was no significant difference in the difference of aBUT, basal tear secretion difference and corneal thickness between before and after wearing overnight orthokeratology(P=0.0922、0.0891、0.4181); there was significant difference in the difference of corneal curvature, corneal thickness, the coefficient of variation of corneal endothelial cell, and the percentage of corneal hexagonal endothelial cells between before and after wearing overnight orthokeratology(P=0.0000、0.0005、0.0002). No serious complications occurred in ocular surface in all follow-up cases.

CONCLUSION: Long-term overnight orthokeratology had no significant effect on tear film stability, but would increase the coefficient of variation of the corneal endothelial cell and reduce the percentage of corneal hexagonal endothelial cells. In order to diminish implications and guarantee the safety of orthokeratology, it should be closely observed, regular care.