目的：探讨甲状腺相关眼病(thyroid associated ophthalmopathy，TAO)患者血清可溶性细胞间黏附分子-1(serum soluble intercellular adhesion molecules -1，sICAM-1)、可溶性血管细胞黏附分子-1(soluble vascular cell adhesion molecule-1，sVCAM-1)及外周血单个核细胞(peripheral blood mononuclear cells，PBMC)中微小RNA-146a的表达及其意义。

方法：选取2014-06/2015-12在我院诊治的TAO患者37例(TAO组)、甲状腺功能亢进不伴有眼病患者40例(非眼病组)、健康人群30例(健康组)，检测各组血清sICAM-1、sVCAM-1及PBMC中微小RNA-146a表达。

结果：TAO组的血清sICAM-1(366.14±67.28μg/L)、sVCAM-1(211.07±27.45μg/L)水平显著高于非眼病组(286.62±51.09μg/L、179.83±25.09μg/L)和健康组(234.51±38.969μg/L、164.51±22.57μg/L)，差异有统计学意义(P<0.05)，TAO组PBMC中微小RNA-146a(0.071±0.016)低于非眼病组(0.381±0.084)和健康组(1.105±0.216)，差异有统计学意义(P<0.05)； 非眼病组的血清sICAM-1、sVCAM-1水平显著高于健康组(P<0.05)，非眼病组PBMC中微小RNA-146a表达低于健康组(P<0.05)。轻度组的血清sICAM-1、sVCAM-1水平显著低于中重度组和极重度组(P<0.05)，轻度组PBMC中微小RNA-146a表达高于中重度组和极重度组(P<0.05)； 中重度组的血清sICAM-1、sVCAM-1水平显著低于极重度组(P<0.05)，中重度组PBMC中微小RNA-146a表达高于极重度组(P<0.05)。

结论：血清sICAM-1、sVCAM-1在TAO患者中高表达，PBMC中微小RNA-146a在TAO患者低表达，并且与患者的病情程度有关。

AIM: To study serum soluble intercellular adhesion molecules -1(sICAM-1), soluble vascular cell adhesion molecule -1(sVCAM-1)and expression of miRNA-146a in peripheral blood mononuclear cells(PBMC)and its significance in patients with thyroid associated ophthalmopathy(TAO).

METHODS: From June 2014 to December 2015 in our hospital, 37 patients with TAO(TAO group), 40 patients with hyperthyroidism without TAO(non eye disease group)and 30 healthy people(control group)were enrolled and the serum concentrations of sICAM-1, sVCAM-1 and expression of miRNA-146a in PBMC were detected.

RESULTS: The serum sICAM-1 and sVCAM-1 of TAO group were 366.14±67.28g/L, 211.07±27.45g/L level was significantly higher than those of non eye disease group(286.62±51.09μg/L, 179.83±25.09μg/L)and healthy group(234.51±38.969μg/L, 164.51±22.57μg/L)(P<0.05). In TAO group, miRNA-146a(0.071±0.016)in PBMC was lower than that in non eye disease group(0.381±0.084)and healthy group(1.105±0.216)(P<0.05). The serum levels of sICAM-1 and sVCAM-1 were significantly higher in non eye disease group than in healthy group(P<0.05), and the expression of RNA-146a in PBMC was lower in the non eye disease group than in the healthy group(P<0.05). The serum levels of sICAM-1 and sVCAM-1 in the mild TAO group were significantly lower than those in the moderate-severe groups and extremely severe group(P<0.05), and the miRNA-146a expression in the mild TAO group was higher than those in the moderate-severe group and extremely severe group(P<0.05). The serum levels of sICAM-1 and sVCAM-1 were significantly lower in the moderate-severe group than those in the extremely severe group(P<0.05), and the expression of miRNA-146a in the moderate -severe group was significantly higher than that in the extremely severe group(P<0.05).

CONCLUSION: Serum sICAM-1, sVCAM-1 in TAO patients is with high expression, miRNA-146a in PBMC in TAO patients with low expression, and related to the degree of patient's condition.

河南省科技发展计划项目(No.132300410106)