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[摘要]
目的：探讨miR-146表达与眼球衰老的相关性，以及年龄相关性黄斑变性(age-related macular degeneration，ARMD)发生过程中miR-146与炎症因子表达的相关性及其可能的机制。
方法：分离鼠龄为2～24mo的小鼠RPE，采用qRT-PCR的方法检测RPE中miR-146a/b和IL-6 mRNA的表达水平。检测来自于ARMD患者眼球中miR-146a及IL-6 mRNA的表达水平。在ARPE-19细胞系中检测miR-146a过表达对IL-6基因表达水平的影响。
结果：miR-146的表达在自然衰老的小鼠RPE中与年龄呈现正相关，而IL-6无变化。在ARMD患者中，miR-146a mRNA表达水平下降，IL-6 mRNA表达水平上升。在人ARPE-19细胞中的实验表明，miR-146a过表达抑制了由TNF-α所诱导的IL-6的表达。
结论：在湿性ARMD中，miR-146a与IL-6 mRNA表达水平有可能与ARMD的发生呈相关性，暗示IL-6和miR46a有可能作为生物分子标志物在未来的ARMD诊断中发挥作用。
[Key word]
[Abstract]
AIM: To discuss the correlation between the expression of miR-146 and aging retinal pigment epithelium(RPE), and to study the relationship between miR-146 and expression of IL-6 in RPE, especially in age-related macular degeneration(ARMD).
METHODS: The expressions of miR-146 and IL-6 were examined in RPE in mice aged from 2mo to 24mo by qRT-PCR. Then, the expressions of miR-146 and IL-6 in RPE of wet ARMD patient were examined also. Finally, the effect of overexpression of miR-146a in APRE-19 cell line on expression of IL-6 was checked.
RESULTS: MiR-146 was positive correlated with age, and the expression of IL-6 had no change in aging RPE. However, the expression of miR-146 decreased and IL-6 increased in RPE of ARMD. In cultured APRE-19 cells, overexpression of miR-146 inhibited the expression of IL-6 induced by TNF-α.
CONCLUSION: Our results suggest that there is a biological correlation among the development of ARMD, expression of IL-6 and miR-146 in aging RPE. It also suggests that, on the one hand, regulation between IL-6 and miR-146 may be important for the clinical treatment, on the other hand, both IL-6 and miR-146 can be potential molecular markers for diagnosing ARMD.
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