[关键词]
[摘要]
体外分离与选择性培养视网膜微血管内皮细胞的方法有多种，其中新报道的组织块培养结合胶原酶消化法因操作简便、经济，获得的内皮细胞纯度较高、活性良好等优点而被广泛应用。受人眼球供体的限制，研究者通常会选用分离与人高度同源的鼠视网膜微血管内皮细胞(retinal microvascular endothelial cell，RMVEC)进行体外实验。本文就目前常用的视网膜微血管内皮细胞体外培养方法从不同动物种属、添加因子、细胞纯化、体外鉴定等方面进行阐述，同时简要说明众多方法在操作过程中的优缺点，为研究人员选择合适的培养方法提供参考。
[Key word]
[Abstract]
There are a variety of ways for isolation and selective cultivation of retinal microvascular endothelial cells in vitro, in which the newly reported tissue culture with collagenase digestion method are widely used for simple, economical, high purity and good activity of obtained endothelial cells. As a result of the limitation of person eye donor, researchers often use rat retinal microvascular endothelial cells that are highly homologous to human beings to experiment in vitro. In this paper, the methods of culturing retinal microvascular endothelial cells in vitro will be elaborated from the aspects of animal species, added factors, cell purification and identification in vitro. At the same time, the advantages and disadvantages of these methods in the process of operation are briefly described, which can provide a reference for the researchers to select suitable cultivation methods.
[中图分类号]
[基金项目]
国家自然科学基金项目(No. 81460685)
