方法：取生长状况良好的RF/6A细胞，分为对照组、低剂量组(0.1μmol/L apelin-13)和高剂量组(1μmol/L apelin-13)。培养细胞24h后采用MTT法检测细胞增殖，细胞划痕法检测细胞迁移，基质胶法检测管腔形成。

结果：不同浓度apelin-13作用24h的细胞增殖强于对照组，差异有统计学意义(P<0.05)，不同浓度apelin-13组RF/6A细胞24h的迁移距离大于对照组，差异有统计学意义(P<0.05)，不同浓度apelin-13组毛细血管样管腔形成数明显多于对照组，差异有统计学意义(P<0.05)，且细胞增殖、迁移和管腔形成均随着apelin-13浓度的升高而增加。

结论：Apelin-13能够明显促进RF/6A细胞的血管生成过程，提示apelin-13是一种促视网膜新生血管形成的重要因子。

METHODS: RF/6A cells in good conditions were administrated with DMSO(the control group), apelin-13 at 0.1μmol/L(low dose group)or apelin-13 at 1μmol/L(high dose group). Cell proliferation, migration and capillary-like tube formation were detected by using the MTT assay, scratch assay and matrigel assay, respectively, at 24h after plating the cells.

RESULTS: Cell proliferation was promoted in both low and high dose apelin-13 groups compared to the control cells(P<0.05); the cell migration distance of both apelin-13 groups was significantly greater than that of the control group(P<0.05); and the number of capillary-like tube structures of both apelin-13 groups was significantly larger than that of the control cells(P<0.05). In addition, cell proliferation, migration and tube formation increased as the concentration of apelin-13 increased.

CONCLUSION: Apelin-13 could obviously promote the angiogenesis capacity of RF/6A cells, suggesting that apelin-13 was an important pro-angiogenic factor in retinal endothelial cells.