方法：SD大鼠60只，随机分为3组(每组20只)：对照组、低剂量组和高剂量组，各组分别应用微量注射器行大鼠眼球玻璃体腔内注射，剂量均为0.3μL，对照组给予9g/L生理盐水、低剂量组给予浓度0.5mg/kg衣霉素、高剂量组给予浓度1.5mg/kg衣霉素进行玻璃体腔内注射。在造模后每天散瞳观察眼底，第1、7、14d 通过OCT、眼底照相、眼底荧光、视网膜电图及HE染色观察不同浓度下视网膜各层形态学变化。

结果：OCT结果提示衣霉素对视网膜形态及结构有损伤作用，呈现出时间-剂量依赖性； 眼底照相结果提示在衣霉素注射2wk后，随着衣霉素浓度的变化，视网膜周边及黄斑区颜色逐渐苍白，视盘区水肿，高剂量组出现视网膜血管变细，视神经萎缩； 荧光素造影结果提示：衣霉素注射玻璃体腔2wk后，视网膜血管功能损伤，逐渐出现周边至中央部血管造影剂渗漏； 电生理表明，衣霉素诱导的视网膜电图紊乱，a波、b波逐渐地振幅降低，甚至变平，具有明显的统计学意义(P<0.05)； 石蜡切片HE染色结果提示衣霉素对视网膜各层的损伤呈现剂量-时间依赖性，与OCT结果相一致。

结论：衣霉素可以通过诱导视网膜损伤模拟视网膜疾病模型，OCT可以动态观察视网膜的损伤改变，作为一种非侵入性检查手段对于评价视网膜损伤具有一定积极意义。

METHODS:Totally 60 SD rats were randomly divided into 3 groups(20 in each group), 0.5mg/kg(in low dose group), 1.5mg/kg(in high dose group)tunicamycin were injected into vitreous cavity and saline(9g/L NaCl)were injected in the same dose as a control group. Changes of retinas were observed by OCT on the 1,7 and 14d after treatment of tunicamycin. Then the rats were sacrificed, retinas were taken out and embedded by the paraffin, tissue sections and the HE staining were performed.

RESULTS:OCT results suggested that tunicamycin played damage effects on retinal morphology and structure which appeared a time- and dose- dependent. Fundus photography results suggested that 2wk after tunicamycin treatments, with the gradually changing of tunicamycin concentration, peripheral retinal and macular region became pale color gradually, edema occurred in optic disk, retinal vessels appeared thinner in the high dose group, optic nerve came out atrophy. Fluorescein angiography confirmed that tunicamycin injection in vitreous cavity 2wk later, retinal vessels injury occurred, resulted in leaking of intravascular contrast agent from peripheral to the central part of the retinas. Electrophysiological data showed that retinal electrogram occurred disorder induced by tunicamycin, such as the amplitude of a wave, b wave decreased gradually, even closed to zero, which was very different from control significantly(P<0.05). HE staining of paraffin sections showed that retina injuries induced by tunicamycin were in dose - time dependent, which was consistent with the results of OCT.

CONCLUSION: Clinical retinal diseases could be simulated by retinal damage animal model induced by tunicamycin treatment. OCT detection offered real-time images of the retinal cross-section, which provided a helpful non-invasive method for detecting and evaluating the retinal damages.