目的：探讨白藜芦醇对高糖环境下视网膜血管内皮细胞线粒体DNA的影响及机制。

方法：人视网膜血管内皮细胞培养于正常或高糖环境，提取细胞基因组DNA，Real-time PCR检测线粒体DNA 拷贝数，研究白藜芦醇对高糖培养的视网膜血管内皮细胞线粒体DNA的影响。通过Western-blot及免疫共沉淀检测线粒体转录因子A(mitochondrial transcription factor A，TFAM)表达及PGC-1α乙酰化。

结果：高糖对视网膜血管内皮细胞线粒体DNA的拷贝数有明显的抑制作用，白藜芦醇能够降低视网膜血管内皮细胞PGC-1α的乙酰化，提高TFAM的表达，增加线粒体DNA的拷贝数。

结论：白藜芦醇可能通过激活PCG-1α-TFAM信号通路改善高糖环境下视网膜血管内皮细胞的线粒体功能。

METHODS: Human retinal vascular endothelial cells were cultured in low glucose or high glucose medium, the genomic DNA was extracted and copy number of mitochondrial DNA was detected by real-time PCR. Effects of resveratrol on the mitochondrial DNA copy in retinal vascular endothelial cells cultured under high glucose medium were studied. The expression of mitochondrial transcription factor A(TFAM)and acetylated proliferator-activated receptor coactivator-1 alpha(PGC-1α)were analyzed by Western blot and coimmunoprecipitation.

RESULTS: High glucose inhibited the copy number of mitochondrial DNA in retinal vascular endothelial cells. However, resveratrol decreased the level of acetylated PGC-1α in retinal vascular endothelial cells, increased the expression of TFAM and the copy number of mitochondrial DNA.

CONCLUSION: Resveratrol may improve mitochondrial function of retinal vascular endothelial cells exposed to a high glucose environment via activation of the PCG-1α-TFAM signaling pathway.