目的：从蛋白分子水平分析天麻钩藤饮抗大鼠视神经夹伤模型视网膜神经节细胞凋亡的机制。

方法：将所有大鼠36只随机分为天麻钩藤饮低剂量组(0.6g/mL，n=6)、中剂量组(1.2g/mL，n=6)、高剂量组(2.4g/mL，n=6)、正常对照组(n=6)、阴性对照组(n=6)和银杏叶片阳性对照组(1.2mg/mL，n=6)共6组，再将30只SPF级雄性Wistar大鼠(除正常对照组外其余五组)制备成视神经夹伤模型。检测N-甲基-D-天冬氨酸2B(N-methyl-D-aspartic acid receptor 2B，NMDA2B)受体蛋白平均灰度值和β-淀粉样蛋白(amyloid β-protein，Aβ)平均灰度值。

结果：天麻钩藤饮低、中、高剂量组NMDA2B受体蛋白平均灰度值较阴性对照组明显减少(P<0.001)，并与阳性对照组比较差异无统计学意义(P=0.320)，正常对照组与阴性对照组具有显著性差异(P<0.001)。天麻钩藤饮中、高剂量组β-淀粉样蛋白平均灰度值较阴性对照组明显减少(P=0.086、0.001)； 低浓度组较阴性对照组减少，但差异无统计学意义(P=0.614)； 其中高剂量组和阳性对照组之间比较，差异无统计学意义(P=0.376)； 正常对照组与阴性对照组比较，差异有统计学意义(P<0.001)。

结论：天麻钩藤饮可以通过下调NMDA2B受体蛋白的表达和控制β-淀粉样蛋白的沉积来减少视网膜神经节细胞的损伤和凋亡，并以此机制对视网膜神经节细胞起到一定的保护作用。

METHODS: Totally 36 participants 36 male Wistar rats were divided randomly into six groups(6 in every group): normal control group, negative control group, Tianma Gouteng Decoction treatment groups(con-centrations were 0.6g/mL, 1.2g/mL, 2.4g/mL respictively)and ginkgo biloba tablets positive control group(concentrations was 1.2mg/mL). Nothing was done in the normal control group. The optic nerve of right eye in the other groups was done with the optic nerve crush model. Normal control group and negative control group was treated only with water. The average grey scale values of the N-methyl-D-aspartic acid receptor 2B(NMDA2B)receptor protein, beta - amyloid protein(Aβ)in the average grey scale values were detected.

RESULTS: The average grey scale value of Tianma Gouteng Decoction in low, medium and high dose groups about NMDA2B receptor protein was significantly less than that of the negative control group(all P<0.001), and there was no significant difference with the positive control group(P=0.092, 0.411, 0.676), the difference between normal control group and negative control group was significant(P<0.001). The high dose group of beta-amyloid's average grey scale value reduced significantly than the negative control group(P=0.030, 0.001). The low dose group than the negative control group was not obviously(P=0.614). The high dose group was not significantly different from the positive control group(P=0.927), the difference between normal control group and negative control group was significant(P<0.001).

CONCLUSION: Tianma Gouteng Decoction can go through the decrease of the NMDA2B receptor protein expression and the control of beta-amyloid deposition to reduce the retinal ganglion cell injury and apoptosis.