目的：观察健康人群强迫睁眼前后Oculus 眼表综合分析仪所测非侵入性泪膜破裂时间和干眼分级有无差异。

方法：前瞻性病例自身对照研究。40例志愿者接受此项检查，分别于强迫睁眼前后行Oculus眼表综合分析仪非侵入性首次泪膜破裂时间(non-invasive first tear film break-up time，NIBUT_f)和非侵入性平均泪膜破裂时间(non-invasive average tear film break-up time，NIBUT_av)检查，仪器自动进行干眼分级。首次检查先右眼后左眼，第二次检查先左眼后右眼。

结果：首次检查眼(即强迫睁眼前)NIBUT_f均值为9.18±5.52s、 NIBUT_av均值为11.74±5.59s，干眼各级所占百分比分别为 43%、37%、20%； 第二检查眼(强迫睁眼后)NIBUT_f均值为8.91±5.54s， NIBUT_av均值为11.76±5.58s， 干眼各级所占百分比分别为 35%、48%、16%。健康人群强迫睁眼前后双眼NIBUT_f 、NIBUT_av和干眼分级比较，差异无统计学意义(t=0.37，P=0.72； t=-0.038，P=0.97； Z=-0.42，P=0.68)。

结论：健康人群强迫睁眼前后眼表综合分析仪所测非侵入性泪膜破裂时间和干眼分级无影响。

METHODS: Prospective case self-control study. Forty normal volunteers(79 eyes)were enrolled. Tear film images were captured, non-invasive first tear film break-up time(NIBUT_f), non-invasive average tear film break-up time(NIBUT_av)and dry eye level were measured by Keratograph 5M automatically before and after forced eye opening in each subject. For the first time, we checked the left eye after the right eye, and the second time checked the right eye after the left eye.

RESULTS: The average of NIBUT_f and NIBUT_av were 9.18±5.52s, 11.74±5.59s respectively and percentage of every level of dry eye were 43%, 37%, 20% respectively before forced eye opening. The average of NIBUT_f and NIBUT_av were 8.91±5.54s, 11.76±5.58s and percentage of dry eye at different levels were 35%, 48%, 16% respectively after forced eye opening. There was no significant difference on NIBUT and dry eye level by Oculus keratograph 5M in normal subjects(t=0.37, P=0.72; t=-0.038, P=0.97; Z=-0.42, P=0.68).

CONCLUSION: There is no influence on NIBUT and dry eye level by detected Keratograph 5M before and after forced eye opening in healthy subjects.