目的：研究准分子激光上皮瓣下角膜磨镶术(LASEK)中丝裂霉素C(MMC)的不同作用时间对角膜内皮细胞的影响。

方法：收集我院行LASEK手术的患者，根据丝裂霉素在角膜基质上的作用时间(t)，将研究对象分为T1、T2两组，其中T1组为15s≤t≤45s，T2组为45s结果：本研究共纳入患者98例196眼，T1与T2组分别纳入98眼。周边角膜内皮细胞的ACA、中央角膜内皮细胞的CV和HEX于术后1wk与术前相比差异有统计学意义(P<0.05)，其余各指标在各随访时间两组间差异无统计学意义(P>0.05)。

结论： LASEK术后短期内出现的中央角膜内皮六边形细胞百分比降低、变异系数升高、周边角膜内皮细胞平均面积增大，均为一过性改变。因此，在合理时间范围内应用MMC，不会对远期角膜内皮状态造成显著影响。

METHODS: Patients who received LASEK were included and divided into Group T1(15s≤t≤45s)and T2(5sRESULTS: A total of 196 eyes of 98 patients were included with 98 eyes in Group T1 and 98 eyes in Group T2. With the exception of peripheral ACA, central CV and HEX in Group T1 and T2, which significantly changed at 1wk after the surgery(P<0.05), there was no significant difference in central and peripheral parameters within both groups or between the groups(P >0.05).

CONCLUSION: Although transient acute changes in central CV and HEX and peripheral ACA were observed, there is no significant effect on the long-term corneal health status when MMC is applied in a reasonable time range in LASEK surgery.