[关键词]
[摘要]
RNA干扰(RNA interference，RNAi)是由双链RNA介导、在转录后水平诱导同源基因的mRNA降解来关闭其基因表达的过程。RNAi技术投入少、周期短、操作简单，被广泛运用于基因治疗和基因功能的研究，几乎成为一种标准化技术工具，在生物医学的研究及应用领域具有广阔的前景。眼球相对密闭且免疫豁免，非常适合应用RNAi进行治疗，尤其在白内障领域，RNAi得到越来越广泛的应用。本文主要介绍RNAi技术的基本原理，并对该技术在白内障研究的应用进展进行综述。
[Key word]
[Abstract]
RNA interference(RNAi)is the post-transcriptional gene silencing based on sequence-specific degradation of mRNA, and triggered by double-stranded RNA.RNAi technology is widely used in gene therapy and research of gene function and almost become a standardized technical tool with very broad prospect. The eyes with immune privilege are very suitable for the treatment of RNAi, which has been widely used for a variety of eye diseases, especially cataract. This paper briefly introduces the basic principle of RNAi technology and summarizes the recent research and application of this technology in cataract.
[中图分类号]
[基金项目]
国家自然科学基金资助项目(No.81500705)
