目的：测定新生血管性青光眼(neovascular glaucoma，NVG)患者血清及房水中IL-6、IL-8、TNF-α的表达水平，并探讨IL-6、IL-8、TNF-α在NVG发生发展中的意义。

方法：采用前瞻性病例分析方法，纳入2015-08/2017-03在我院诊治的NVG患者38例38眼，并按虹膜新生血管分级标准分为Ⅱ级8眼，Ⅲ级19眼，Ⅳ级11眼。选取同期原发性开角型青光眼(primary open-angle glaucoma，POAG)患者31例31眼、年龄相关性白内障患者33例33眼作为对照。分别于术前检测患者眼压水平，分别抽取患者静脉血及房水样本，利用酶联免疫吸附法(ELISA)检测血清及房水中IL-6、IL-8、TNF-α含量。

结果：NVG组血清和房水中IL-6、IL-8、TNF-α水平均明显高于POAG组和白内障组，差异有统计学意义(P<0.05)； POAG组血清和房水中IL-6、IL-8、TNF-α水平显著高于白内障组(P<0.05)。Ⅳ级NVG组血清和房水中IL-6、IL-8、TNF-α水平显著高于Ⅲ级患者，差异有统计学意义(P<0.05)，Ⅲ级患者血清及房水中IL-6、IL-8、TNF-α水平明显高于Ⅱ级，差异有统计学意义(P<0.05)。NVG患者血清及房水中IL-6、IL-8、TNF-α水平均与眼压水平呈正相关(P<0.05)。

结论：IL-6、IL-8、TNF-α在NVG患者血清及房水中高表达，可能参与虹膜新生血管生长和眼压升高。

METHODS:A prospective case analysis method was applied to include patients with neovascular glaucoma in 38 cases(38 eyes), and according to grading criteria of iris neovascularization, they were divided into grade Ⅱ with 8 eyes, grade Ⅲ with 19 eyes, grade Ⅳ with 11 eyes. Thirty-one patients(31 eyes)with primary open angle glaucoma(POAG)and 33 patients(33 eyes)with age related cataract were selected as the control. IOP level was detected preoperatively, and venous blood and aqueous humor samples of patients were selected, and IL-6, IL-8 and TNF-α contents in serum and humor were detected by using enzyme linked immunosorbent assay(ELISA).

RESULTS:IL-6, IL-8 and TNF-α levels in serum and aqueous humor of NVG group were significantly higher than those in POAG group and cataract group(P<0.05). IL-6, IL-8 and TNF-α levels in serum and aqueous humor of POAG group were significantly higher than those in cataract group(P<0.05). IL-6, IL-8 and TNF-α levels in serum and aqueous humor of grade ⅣNVG group were significantly higher than those of patients with grade Ⅲ(P<0.05). IL-6, IL-8 and TNF-α levels in serum and aqueous humor of patients with grade Ⅲ were significantly higher than those of grade Ⅱ(P<0.05). IL-6, IL-8 and TNF-α levels in serum and aqueous humor of NVG patients were positively correlated with IOP(P<0.05).

CONCLUSION: IL-6, IL-8 and TNF-α are highly-expressed in serum and aqueous humor of NVG patients. It may be involved in iris neovascularization and intraocular pressure elevation.