目的：探讨石斛酚对高糖诱导的人视网膜微血管内皮细胞(HRMECs)的醛糖还原酶(AR)通路及氧化应激的保护作用。

方法：将体外培养的HRMECs分为5组：正常组(葡萄糖5.5mmol/L)、甘露醇组(葡萄糖5.5mmol/L +甘露醇24.5mmol/L)、高糖组(葡萄糖30mmol/L)、石斛酚组(葡萄糖30mmol/L+石斛酚5μmol/L)、依帕司他组(葡萄糖30mmol/L+依帕司他1μmol/L)。通过MTS实验检测细胞活力，DCFH-DA荧光探针法检测细胞内活性氧(ROS)水平； Western blotting法检测核因子κB(NF-κB)的蛋白表达； qRT-PCR法检测AR、HIF-1α及VEGF的mRNA表达。

结果：DCFH-DA荧光探针法结果显示石斛酚可明显降低高糖环境下细胞内的ROS水平。与高糖组相比，石斛酚组NF-κB的蛋白表达，AR、HIF-1α及VEGF的mRNA表达也都明显下降。依帕司他组的AR及NF-κB表达趋势与石斛酚组一致。

结论：石斛酚与依帕司他都可抑制AR/NF-κB通路的激活，同时石斛酚还可抑制高糖诱导的HRMECs氧化应激反应。石斛酚在糖尿病视网膜病变的发生发展中可能有一定的保护作用，可能帮助减少新生血管的生成。

METHODS: HRMECs were cultured and divided into the five groups: normal glucose group, mannitol group,high glucose group, gigantol group and epalrestat group. Cell vitality was detected by the MTS assay, reactive oxygen species(ROS)levels were detected by DCFH-DA fluorescent probe, protein expressions of nuclear factor kappa B(NF-κB)were observed by Western blotting, mRNA levels of AR, hypoxia inducible factor-1α(HIF-1α)and vascular endothelial growth factor(VEGF)were measured by qRT-PCR.

RESULTS: DCFH-DA fluorescent probe levels showed that gigantol reduced ROS generation obviously, and protein expressions of NF-κB, mRNA expressions of AR,HIF-1α and VEGF were all decreased. The expression trends of AR and NF-κB in epalrestat group were consistent with gigantol group.

CONCLUSION: Gigantol and epalrestat could inhibit the activation of AR/NF-κB pathway, and gigantol also could inhibit high glucose induced oxidative stress and in HRMECs, which may have a protective effect on diabetic retinopathy(DR), and improve angiogenesis.