目的：探讨阿柏西普对大鼠视网膜Müller细胞信号转导及转录活化因子3(STAT3)表达的影响。

方法：采用不同浓度的阿柏西普 100μL(加药浓度分别为400、200、100pg/mL)大鼠永生化视网膜Müller细胞24、48h后，MTT法检测细胞增殖，流式细胞仪检测细胞凋亡，Transwell小室法检测细胞侵袭，Western-blot法检测AKT、STAT3蛋白表达。

结果：随着阿柏西普浓度的升高，Müller细胞的增殖活性下降(P<0.05)。处理48h后，随着阿柏西普浓度的升高，Müller细胞的凋亡率逐渐上升，Müller细胞的侵袭穿透指数逐渐降低(P<0.05)。转染48h后，随着阿柏西普浓度的升高，Müller细胞的AKT蛋白相对表达量无显著变化(P>0.05)，STAT3蛋白相对表达量逐渐降低(P<0.05)。

结论：阿柏西普可抑制大鼠视网膜Müller细胞STAT3蛋白表达，从而抑制细胞增殖与侵袭性，促进细胞凋亡。

METHODS: The rat retinal Müller cells treated with different concentrations of Aflibercept 100 μL(diluted concentrations of 400, 200, 100 pg/mL, respectively). MTT assay were used to detect cell proliferation, flow cytometry. Apoptosis were detected by the instrument, cell invasion were detected by transwell chamber method, and protein(AKT, STAT3, GAPDH)expression were detected by Western-blot method.

RESULTS: The proliferation activity of Müller cells were decreased with the increased of aflibercept concentration, and compared the difference were statistically significant(P<0.05). After treatment for 48h, the apoptotic rate of Müller cells were gradually increased with the increased of aflibercept concentration, and the invasion and penetration index of Müller cells gradually were decreased, and compared the difference were statistically significant(P<0.05). After 48 h of transfection, the relative expression of AKT protein in Müller cells were not change significantly with the increased of Aflibercept concentration(P>0.05), and the relative expression of STAT3 protein decreased gradually, and compared the difference were statistically significant(P<0.05).

CONCLUSION: Aflibercept can inhibit the expression of STAT3 protein in rat retinal Müller cells, thereby inhibit cell proliferation and invasion and promote apoptosis.