目的：研究黄连素对体外高糖环境下的SD大鼠视网膜Müller细胞增殖的影响。

方法：采用酶消化法原代培养SD大鼠视网膜Müller细胞，将第2代Müller细胞随机分为正常糖浓度(5mmol/L)组、高糖浓度(25mmol/L)组、高糖+不同浓度(5、10、25、50、100μmol/L)黄连素组，分别培养24、48、72h后，采用CCK-8法检测细胞增殖活性。

结果：培养24、48、72h时，高糖浓度组Müller细胞吸光度值较正常糖浓度组明显降低(均P<0.01)； 10、25、50、100μmol/L黄连素组细胞吸光度值较高糖浓度组明显升高(均P<0.05)，且呈剂量依赖性； 5μmol/L黄连素组细胞吸光度值与高糖浓度组无明显差异(P>0.05)。

结论：黄连素在一定程度上能够减轻高糖对Müller细胞增殖活性的抑制作用，其作用强度与黄连素浓度呈正相关。

METHODS: The retinal Müller cells of SD rats were primary cultured by enzyme digestion. The second generation of Müller cells were randomly divided into 7 groups. They were normal glucose concentration(5mmol/L glucose)group, high glucose concentration(25mmol/L glucose)group, high glucose+ berberine group(5, 10, 25, 50 and 100μmol/L). After cultured for 24h, 48h and 72h, the cell proliferative viability was measured by CCK-8 method.

RESULTS: After cultured for 24h, 48h and 72h, compared to the normal glucose concentration group, the absorbance of cells in the high glucose concentration group reduced significantly(All P<0.01). Compared to the high glucose concentration group, the absorbance of cells in different concentration berberine(10, 25, 50 and 100μmol/L)groups increased significantly(All P<0.05). It showed a dose-dependent effect. There was no statistically significant difference on the cells absorbance between high glucose+5μmol/L berberine group and high glucose group(P>0.05).

CONCLUSION: Berberine could reduce the inhibitory effect of high glucose on the proliferative viability of Müller cells to some extent. The intensity of effect was positively correlated with the berberine concentration.